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United States Department of Agriculture

Agricultural Research Service

Research Project: Innovative Bioresource Management Technologies for Enhanced Environmental Quality and Value Optimization

Location: Coastal Plain Soil, Water and Plant Conservation Research

Title: Enhanced deammonification of livestock wastewater using Brocadia caroliniensis and HPNS in single tank process

item Vanotti, Matias
item Nishiyama, Takaski
item Martinez, Jose
item Kunz, Airton
item Fujii, Takao
item Furujawa, Kenji

Submitted to: Meeting Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 5/10/2013
Publication Date: 6/11/2013
Citation: Vanotti, M.B., Nishiyama, T., Martinez, J., Kunz, A., Fujii, T., Furujawa, K. 2013. Enhanced deammonification of livestock wastewater using Brocadia caroliniensis and HPNS in single tank process. In: Proceedings of the 2nd International Anammox Symposium, June 11-12, 2013, Coex, Seoul, Korea, p. 39-40.

Interpretive Summary:

Technical Abstract: In this work we describe new findings that allowed rapid implementation of deammonification reaction in livestock anaerobic digestion effluents using mixtures of two bacterial cultures and a one-stage process (partial nitritation and anammox in a single tank). The bacterial cultures were high performance nitrifying sludge (HPNS) accession number NRRL-B-50298 and anammox bacterial sludge, Brocadia caroliniensis, accession number NRRL B-50286. The single-tank reactors were tested with digested swine wastewater. The reactors contained biofilm plastic carriers (30-40% volume/volume) that were fluidized by aeration. The process water temperature was ambient 23±2 degrees Celsius. Surprisingly, the two bacteria groups were able to associate quickly and effectively in the aerated single tanks providing a streamlined ammonia removal process. Microbial reverse transcription analyses indicated that bacteria in the digested swine influent had little effect on the bacterial community that was active in the single-tank. Results showed physiologically high activity of ammonia oxidizing bacteria (AOB) and anammox bacteria in the reactor. High rates of ammonia removal were obtained in a single tank (~ 1.0 kilogram nitrogen per cubic meter of reactor per day) with ammonia removal efficiency of 100%. Compared with traditional Nitrogen removal, the deammonification process reduced 56-57% of the aeration. The nitrogen was removed in a single-tank, further reducing equipment costs. Therefore, deammonification is a key technology for development of more economical and energy efficient biological ammonia removal systems.

Last Modified: 10/20/2017
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