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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Crop Improvement and Genetics Research » Research » Publications at this Location » Publication #293762

Title: Founder lines for improved citrus biotechnology

item Thomson, James - Jim
item Stover, Eddie

Submitted to: Citrograph
Publication Type: Trade Journal
Publication Acceptance Date: 12/20/2012
Publication Date: 1/1/2013
Citation: Thomson, J.G., Stover, E.W. 2013. Founder lines for improved citrus biotechnology. Citrograph. 38-39.

Interpretive Summary: In layman terms this article details how recombinases work, how they can be used for precise genome engineering and why this is a benefit. The article goes further to explain how a specific recombinase strategy will be used for genome targeting that will provide the gene of interest and remove excess DNA such as selectable markers.

Technical Abstract: This article discusses the research needed to develop the RMCE strategy and molecular assays for site-specific recombinases as tools for genome manipulation. Explanation of genetic engineering used to generate transgenic citrus plants to exhibit a novel phenotype, but not to contain the recombinase or selectable marker DNA in a single generation are detailed.