Location: Animal Parasitic Diseases LaboratoryTitle: An anti-tumor protein produced by Trichinella spiralis and identified by screening a T7 phage display library, induces apoptosis in human hepatoma H7402 cells Author
Submitted to: Veterinary Parasitology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/19/2013
Publication Date: 5/20/2013
Citation: Wang, X.L., Liu, M.Y., Sun, S.M., Liu, Y., Wang, X.R., Chu, L.X., Rosenthal, B.M., Shi, H.N., Boireau, P., Wang, F., Zhao, Y., Wu, X.P. 2013. An anti-tumor protein produced by Trichinella spiralis and identified by screening a T7 phage display library, induces apoptosis in human hepatoma H7402 cells. Veterinary Parasitology. 194:186-188. Interpretive Summary: Parasites are known to powerfully influence the immune system and cell signalling processes in their hosts. Interestingly, infection with Trichinella spiralis has been shown to slow or stop the growth of certain cancerous tumors. Understanding the mechanisms by which cancer can be opposed by such parasites, or products that such parasites excrete, could represent an important step forward in understanding and combating cancer. Here, candidate parasite proteins secreted by Trichinella spiralis were screened in cell proliferation assays and assays for programmed cell death. One gene product was found to induce the death of human hepatoma cells. The potential for this and related parasite products as an anti-cancer therapy warrants further study.
Technical Abstract: Trichinella spiralis infection confers effective resistance to tumor cell expansion. In this study, a T7 phage cDNA display library was constructed to express genes encoded by T. spiralis. Organic phase multi-cell screening was used to sort through candidate proteins in a transfected human chronic myeloid leukemia cell line (K562)and a human hepatoma cell line (H7402) using the display library. The protein encoded by the A200711 gene was identified and analyzed using protein analysis software. To test the antitumor effects of A200711, variations in cell proliferation and apoptosis were monitored after recombinant pEGFP-N1-A200711 was transfected into H7402 cells. The results show that the expressed target gene successfully induced apoptosis in H7402 cells as measured by Hoechst-PI staining, MTT assay (p<0.05). This study warrants further investigation into the therapeutic use of A200711 for anti-hepatocellular carcinomas.