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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #293445

Title: Proteomic study of soybean and nematode interaction

item Natarajan, Savithiry - Savi

Submitted to: Plant Protection Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/22/2013
Publication Date: 6/1/2013
Citation: Natarajan, S.S. 2013. Proteomic study of soybean and nematode interaction. Plant Protection Science . 4(1):57-59.

Interpretive Summary: The soybean cyst nematode (SCN) is the most devastating pest of soybean in the United States. To improve soybean yields through an increased level of plant resistance to targeted pests, it is important to understand the protein composition of the SCN. In this review, we discuss the separation and identification of SCN proteins in SCN susceptible and resistant soybean. The results of this study will be useful to scientists who wish to develop nematode resistant soybeans.

Technical Abstract: Soybean cyst nematode (SCN), Heterodera glycines, is a major pathogen of soybean. In order to devise strategies to control SCN and develop soybeans with broad resistance against SCN, basic information on the nematode’s proteome is essential to have a better understanding of SCN parasitism and its virulence. Genomic and proteomic approaches have been successfully used to study plant pathogen interactions. In this review, we discussed proteomic analysis of SCN and its pathogenicity. Differential proteome analysis of resistant and sensitive soybeans infected by SCN using two dimensional gel electrophoresis (2D-PAGE) and mass spectrometry (MS) found 27 differentially expressed proteins. The majority of these proteins were involved in defense, energy and metabolism. The identification of protease inhibitors plays a role in regulating endogenous proteases during development and defense of plants against insects and nematode attack. In our lab, we used a proteomic approach to analyze differentially expressed proteins in 3 days post infected soybean root. We concluded that, the combined application of different molecular technologies such as genomics and proteomics will provide much needed information about the interaction of nematodes with soybeans, and the differences in response of resistant and susceptible varieties of soybeans to nematode infection.