|HE, G - Tuskegee University|
|ZHAO, Y - Tuskegee University|
|YUAN, M - Shandong Peanut Research Institute|
Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2013
Publication Date: 7/11/2013
Citation: He, G.H., Barkley, N.L., Zhao, Y.L., Yuan, M. 2013. Use of EST-SSR loci flanking regions for phylogenetic analysis of genus Arachis. American Peanut Research and Education Society Abstracts. Paper No. 93.
Technical Abstract: All wild peanut collections in the genus Arachis were assigned to nine taxonomy sections on the bases of cross-compatibility and morphologic character clustering. These nine sections consist of 80 species from the most ancient to the most advanced, providing a diverse genetic resource for phylogenetic study in Arachis. However, limited information on phylogenetic relationships among these species from all nine sections at the molecular level is available. In this study, seven gene-based markers (EST-SSRs) were employed to assess divergence and genome affinities between 17 Arachis species drawn from nine taxonomic sections, to infer phylogenetic relationships and elucidate the genome evolution in the genus Arachis by comparative analysis of gene sequence data. Sequences of seven gene homologs showed that variations within SSR and flanking region provide informative data on reconstructing the phylogeny of Arachis. The analysis revealed the concatenated gene tree was generally congruent with the Arachis species tree. The section Arachis formed a single group in which A. glandulifera with DD genome and A. ipaensis with BB genome clustered together forming one subgroup, while A. duanensis, A. diogoi, and A. stenosperma with AA genome were clustered into another subgroup. Species from other sections were grouped coincidently with cross-compatibility, suggesting their genome affinities.