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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Poultry Microbiological Safety and Processing Research Unit » Research » Publications at this Location » Publication #292633

Title: Enhancing Aerobic Growth of Campylobacter in Media Supplemented with Organic Acids

item Hinton, Jr, Arthur

Submitted to: Campylobacter Helicobacter and Related Organisms International Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 6/5/2013
Publication Date: 9/5/2013
Citation: Hinton Jr, A. 2013. Enhancing Aerobic Growth of Campylobacter in Media Supplemented with Organic Acids. Society for General Microbiology. p. 27.

Interpretive Summary: n/a

Technical Abstract: The effect of agar and sodium bicarbonate (NaHCO3) concentration on aerobic growth of Campylobacter in was determined. A fumarate-pyruvate medium was supplemented with 0.0 to 0.2% agar and inoculated with Campylobacter coli, Campylobacter fetus, or Campylobacter jejuni. Portions of the inoculated media were transferred to wells of a honeycomb plate of a Bioscreen Microbiology Reader. The optical density (OD) of cultures was measured during aerobic incubation at 37C for 72 h. Next, fumarate-pyruvate media containing 0.15% agar was supplemented with 0.00 to 0.10% NaHCO3 and inoculated with Campylobacter then growth was measured as previously described. Experiments were then conducted to compare the number of Campylobacter recovered from media supplemented with 0.15% agar and 0.05 % NaHCO3 and incubated aerobically or microaerophilically for 72 h at 37C. Results of experiments indicated that the OD of cultures of all Campylobacter isolates was significantly higher when grown in fumarate-pyruvate broth media supplemented with agar. Furthermore, addition of NaHCO3 to the medium supplemented with agar produced a significant increase in the OD of most isolates. Also, after 72 of incubation there was a 5 to 6 log increase in Campylobacter recovered from inoculated media containing 0.15% agar and 0.05% NaHCO3, and there was no significant difference in the number of CFU recovered from media incubated aerobically or microaerophilically. Findings indicate that supplementing fumarate-pyruvate broth medium with agar and NaHCO3 produces significant increases aerobic growth of Campylobacter. This medium might provide a less expensive, simplified alternative to current procedures for culturing Campylobacter under microaerophilic conditions.