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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #292383

Title: Serotype assignment by sero-agglutination, ELISA, and PCR

item Gorski, Lisa

Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 10/23/2013
Publication Date: 5/20/2014
Citation: Gorski, L.A. 2014. Serotype assignment by sero-agglutination, ELISA, and PCR.In: Jordan, K., Fox, E.M., Wagner, M., editors. Methods in Molecular Biology. New York, NY: Humana Press. p. 41-61.

Interpretive Summary:

Technical Abstract: For assessing isolates of Listeria monocytogenes serotype designation is the foremost subtyping method used. Traditionally serotyping has been done with agglutination reactions. In the last decade alternative serotyping methods were described using Enzyme Linked Immunosorbent Assay(ELISA)and Polymerase Chain Reaction(PCR). Herein are described the three methods, and the advantages and disadvantages of the three.