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Title: USE OF INTRAGENIC SEQUENCE RIBOTYPING (ISR) FOR SEROTYPING Salmonella OBTAINED FROM COMMERCIAL BIRDS AND POULTRY ENVIRONMENT IN MISSISSIPPI AND DETERMINATION OF THEIR ANTIBIOTIC RESISTANCE PATTERNS.

Author
item PULIDO-LANDINEZ, MARTHA - University Of Colombia
item WASHINGTON, PAUL - Mississippi State University
item THORTON, JAY - Mississippi State University
item BANDA, ALEJANDRO - Mississippi State University
item SANCHEZ-INGUNZA, R - Former ARS Employee
item Guard, Jean
item DO NASCIMENTO, VLADIMIR - Federal University Of Rio Grande Do Sul
item MAUEL, MICHAEL - Mississippi State University

Submitted to: American Association of Avian Pathologist
Publication Type: Abstract Only
Publication Acceptance Date: 3/11/2013
Publication Date: 7/22/2013
Citation: Pulido-Landinez, M., Washington, P., Thorton, J., Banda, A., Sanchez-Ingunza, R., Guard, J.Y., Do Nascimento, V., Mauel, M. 2013. USE OF INTRAGENIC SEQUENCE RIBOTYPING (ISR) FOR SEROTYPING Salmonella OBTAINED FROM COMMERCIAL BIRDS AND POULTRY ENVIRONMENT IN MISSISSIPPI AND DETERMINATION OF THEIR ANTIBIOTIC RESISTANCE PATTERNS. American Association of Avian Pathologist. p. 13.

Interpretive Summary:

Technical Abstract: The PCR method called Intergenic Sequence Ribotyping (ISR), detects the region neighboring the gene dkgB from the end of the 23S gene to the start of the gene tRNA aspU. It is hypothesized that the ISR may be a useful method to genotype Salmonella enterica. The purpose of this research is to compare the results obtained by ISR with those obtained with the traditional Kauffman-White-Le Minor (KWL) scheme (Performed by NVSL, Ames, IA) for the characterization and serotyping of Salmonella isolates from commercial birds and their environment included in the bacterial collection of the Poultry Research and Diagnostic Lab (PRDL). Furthermore, considering that the acquisition of antimicrobial resistance of Salmonella from poultry is a serious public health issue and that the National Antimicrobial Resistance Monitoring System (NARMS) establishes Salmonella as the sentinel organism of this system, in this study the Antibiotic Resistance Patterns (ARP) of selected S. enterica isolates (Enteritidis, Kentucky, Typhimurium, Montevideo, Mbandaka, 4,5,12 i:-, Bredeney and Saintpaul) were determined by Minimum Inhibitory Concentration (Sensititre Microbiologic Systems). By using ISR it was possible to serotype 50 Salmonella enterica isolates, the results determined by ISR were in agreement with KML in more than 92% of the cases. It was also possible to identify the possible presence of mixed serotypes. The antibiotic susceptibility test showed differences in ARP between Salmonella serotypes and within isolates of the same serotype. Results may be updated to include additional data prior to presentation.