Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/13/2013
Publication Date: 3/29/2013
Citation: Ridpath, J.F., Fulton, R.W., Burge, L., Neill, J.D. 2013. Genotyping bovine coronaviruses[Abstract]. In: Proceedings of 7th Biennial All Iowa Virology Symposium, March 20-30, 2013. Ames, Iowa. p.31. Interpretive Summary:
Technical Abstract: Bovine coronaviruses (BoCV) are enveloped, single-stranded, positive-sense RNA viruses of the Coronaviridae family. Infection is associated with enteritis and pneumonia in calves and Winter Dysentery in adult cattle. Strains, isolated more than 50 years ago, are used in vaccines and as laboratory reference strains. Phylogenetic comparison, based on spike protein coding sequences, of these strains with more recently isolated field strains was conducted. Four genotypes were identified; one was composed of strains isolated prior to 1970 from cattle in the U.S. and Canada, one of isolates from Australia, one of isolates from dairy calves from the northeastern U.S. and one was isolates from beef cattle from southwestern U.S. feedlots. The southwestern U.S. isolates could be further divided into 3 subgroups. Phylogenetic analysis was also done with a select number of the isolates based on comparison of other structural proteins and the complete genome. A significant deletion was detected in the genome of a virus used in an attenuated vaccine. Genetic differences could be correlated with antigenic differences. These results suggest that the BoCV isolates circulating in the U.S. have changed over the last half century. This observation impacts the selection of vaccine and reference strains.