Location: Avian Disease and Oncology ResearchTitle: Temporal changes of microRNA gga-let-7b and gga-let-7i expression in chickens challenged with subgroup J avian leukosis virus
|JI, JUN - South China Agricultural University|
|SHUANG, H - South China Agricultural University|
|LI, H - South China Agricultural University|
|CHANG, SHUANG - Michigan State University|
|MA, J - South China Agricultural University|
|BI, Y - South China Agricultural University|
|XIE, QINGMEI - South China Agricultural University|
Submitted to: Veterinary Research Communications
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/8/2017
Publication Date: 2/11/2017
Publication URL: http://handle.nal.usda.gov/10113/5700720
Citation: Ji, J., Shuang, H., Zhang, H., Li, H., Chang, S., Ma, J., Bi, Y., Xie, Q. 2017. Temporal changes of microRNA gga-let-7b and gga-let-7i expression in chickens challenged with subgroup J avian leukosis virus. Veterinary Research Communications. doi:10.1007/s11259-017-9681-1.
Interpretive Summary: MicroRNAs are a class of small non-coding RNA molecules about 21-22 nucleotides in length. MicroRNAs are commonly found in both plants and animals, which affect gene functions by regulating the process from genes to gene products. Two of the important microRNAs, namely gga-let-7b and gga-let-7i, were examined in chickens challenged with avian leukosis viruses. Our data suggested that both of the microRNAs may suppress tumor formation in chicken. This finding may help to develop genetic control measures to prevent avian leukosis in chickens.
Technical Abstract: Two important microRNAs, gga-let-7b and gga-let-7i were examined for the relative expression in liver and bone marrow tissues from specific pathogen free chickens that were challenged either with GD1109 or NX0101 strain of subgroup J avian leukosis virus (ALV-J). The GD1109 strain of ALV-J reportedly causes hemangioma (HE) and NX0101 reportedly causes myeloma (ML) in susceptible chickens. Temporal changes of both gga-let-7b and gga-let-7i expression in ALV-J infected chickens were observed in contrast to its counterpart of a non-infected negative control group of chickens (P < 0.05 or P < 0.01) during the first 120 days post infection. Use of the web-based computational DIANA-mirPath software(available at http://microrna.gr/mirpath), it was predicted that both gga-let-7b and gga-let-7i were involved in multiple pathways including signaling pathways, such as MAPK, TGF-beta, Notch, Wnt,mTOR, Cell cycle, P53 and Jak-STAT. Combining our experimental data with reports on the microRNAs, we suggest that both gga-let-7i and gga-let-7b may also act as tumor suppressors in chicken, especially play a critical role in tumorigenesis induced by ALV-J.