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Title: Complete genome sequence of sweet potato latent virus and its relationship to other potyviruses infecting sweet potato

item WANG, MINGQIANG - South China Agricultural University
item ABAD, JORGE - Animal And Plant Health Inspection Service (APHIS)
item FUENTES, SEGUNDO - International Potato Center
item Li, Ruhui

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/23/2013
Publication Date: 5/1/2013
Citation: Wang, M., Abad, J., Fuentes, S., Li, R. 2013. Complete genome sequence of sweet potato latent virus and its relationship to other potyviruses infecting sweet potato. Archives of Virology. DOI: 10.1007/s00705-013-1705-8.

Interpretive Summary: Sweet potato [Ipomoea batatas (L.) Lam] is an important root/tuber crop worldwide, especially in Africa and Asia. Mix infections of several viruses such as Sweet potato latent virus (SPLV) cause sweet potato viral disease (SPVD). SPVD is one of the most devastating sweet potato diseases worldwide and can reduce the yields up to 80-90%. In this study, the complete genomic sequence of SPLV was determined for the first time. Analyses of the genetic information show that this is a distinct species in a virus genus named Potyvirus. The study provides the information necessary for the proper grouping and naming of the virus. This research will also facilitate the development of fast and reliable diagnostic methods for the virus.

Technical Abstract: The complete genome of sweet potato latent virus (SPLV) was determined to be 10,081 nucleotides long, excluding the 3’ poly (A) tail. The genome contains a single large open reading frame encoding a polyprotein of 3,247 amino acids. Its genomic organization is typical of potyviruses and contains motifs conserved in members of the genus Potyvirus. Pairwise comparisons show that SPLV shares identities of 50.0%-56.3% to other potyviruses at the genomic sequence level. Phylogenetic analysis shows that SPLV is most closely related to four other sweet potato potyviruses in sweet potato feathery mottle virus lineage, but it lacks the unique PISPO in P1 region of those viruses. The genome analyses confirm that SPLV is a distinct sweet potato virus in the genus Potyvirus.