|CAO, GUANGLI - Harbin Institute Of Technology (HIT)|
|XIMENES, EDUARDO - Purdue University|
|LADISCH, MICHAEL - Purdue University|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/29/2013
Publication Date: 4/30/2013
Citation: Cao, G., Ximenes, E., Nichols, N.N., Ladisch, M. 2013. Biological abatement of enzyme inhibitors [abstract].
Technical Abstract: Lignocellulose pretreatments release phenolic compounds that cause enzyme inhibition and deactivation. Bio-abatement, the biological removal of furfurals, acetic acid and phenolics, may utilize fungal fermentation to metabolize these compounds to CO2, water, cell mass, and heat. Our work with Coniochaeta ligniaria shows a beneficial effect occurs due to removal of inhibitors. Fermentation of liquid obtained from dilute acid pretreatment of corn stover removed all of the acetic acid and between 30 to 95% of formic acid, HMF, furfural, and phenolic compounds in 48 hours. Higher cellulose conversions were observed for bio-abated compared to untreated liquid samples. Hydrolysis was carried out with solka floc (cellulose) at either 3.6 mg cellulase protein/g cellulose or 36 mg protein/g. When a bio-abated liquid from liquid hot water pretreated corn stover was added to solka floc, enzyme hydrolysis gave 67% yield of glucose while conversion in non-bio-abated liquid was 52% at 200 g/L pretreated, not washed solids.