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Title: Isolation and characterization of microsatellite Loci for Cornus sanguniea (Cornaceae) 1

item WADL, PHILLIP - University Of Tennessee
item HATMAKER, ANNIE - University Of Tennessee
item FUSSI, BARBARA - Non ARS Employee
item Scheffler, Brian
item TRIGIANO, ROBERT - University Of Tennessee

Submitted to: American Journal of Botany
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/1/2013
Publication Date: 9/13/2013
Citation: Wadl, P.A., Hatmaker, A., Fussi, B., Scheffler, B.E., Trigiano, R.N. 2013. Isolation and characterization of microsatellite Loci for Cornus sanguniea (Cornaceae) 1. American Journal of Botany. 1(9):1300012. doi:10.3732/apps.1300012.

Interpretive Summary: Cornus sanguineais is a species of USDA dogwood native to most of Europe and Asia. It is an ornamental plant that is easy to grow up to zone 5, with an estimated height of 9-10 feet. The shrub can be planted in a hedge style formation or stand alone. Little is known about the genetic diversity of sanguineais populations and since it is often propagated as clones the species could be genetically vulnerable to changing environmental conditions or new pests. This study developed DNA markers for future experiments looking at the genetic diversity of sanguineais populations.

Technical Abstract: Premise of the study: Microsatellite loci were developed for Cornus sanguinea and will permit genetic and conservation studies of the species. Methods and Results: A microsatellite-enriched library was used to develop 16 polymorphic microsatellite loci for C. sanguinea. The loci amplified 5-11 alleles per locus. Observed heterozygosity ranged from 0.00 to 1.00, expected heterozygosity ranged from 0.65 to 0.90, and polymorphic information content ranged from 0.59 to 0.88. Conclusions: These microsatellite loci provide a set of markers for further investigation of population genetics of C. sanguinea.