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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #289132

Title: Quantification and characterization of mucosa-associated and intracellular Escherichia coli in inflamatory bowel disease

item ELLIOTT, TIMOTHY - King'S College
item HUDSPITH, BARRY - King'S College
item WU, GUANGHUI - Veterinary Laboratories Agency (VLA)
item Cooley, Michael - Mike
item PARKES, GARETH - King'S College
item Quiñones, Beatriz
item RANDALL, LUKE - Veterinary Laboratories Agency (VLA)
item Mandrell, Robert
item Fagerquist, Clifton - Keith
item BROSTOFF, JONATHAN - King'S College
item RAYMENT, NEIL - King'S College
item BOUSSIOUTAS, ALEX - University Of Melbourne
item PETROVSKA, LILJANA - Veterinary Laboratories Agency (VLA)
item SANDERSON, JEREMY - King'S College

Submitted to: Inflammatory Bowel Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/13/2013
Publication Date: 8/28/2013
Publication URL: http://doi: 10.1097/MIB.0b013e3182a38a92
Citation: Elliott, T.R., Hudspith, B.N., Wu, G., Cooley, M.B., Parkes, G., Quinones, B., Randall, L., Mandrell, R.E., Fagerquist, C.K., Brostoff, J., Rayment, N.B., Boussioutas, A., Petrovska, L., Sanderson, J.D. 2013. Quantification and characterization of mucosa-associated and intracellular Escherichia coli in inflamatory bowel disease. Inflammatory Bowel Diseases. 19(11):2326-2338.

Interpretive Summary: The role of Escherichia coli (E. coli) in the pathogenicity of Crohn's disease (CD) is studied by characterizing E. coli from mucosal biopsies of CD and ulcerative colitis patients and healthy controls. The lack of characterising pathogenic features among E. coli from these different groups suggests that a defective mucosal immunity may be responsible for intracellular access of E. coli.

Technical Abstract: Background and aims: Mucosa-associated E. coli are abundant in Crohn’s disease (CD) but whether these bacteria gain intracellular access within the mucosa is less certain. If E. coli does gain intracellular access in CD, the contribution of bacterial pathogenicity as opposed to a defect in host innate immunity to this requires further elucidation. This study aimed to quantify and characterise mucosa-associated and intracellular E. coli in CD and controls. Methods: Mucosal biopsies from 30 patients with CD, 15 with ulcerative colitis (UC) and 14 healthy controls were cultured with or without gentamicin protection to recover intracellular or mucosa-associated E. coli respectively. Forty of these strains (CD (n = 24), UC (n = 9) and HC (n = 7)) were characterised by phylogenetic typing, adhesion and invasion assays, detection of virulence factors, antimicrobial resistance(AMR)genes and proteomic analysis. Results: Mucosa-associated E. coli were more abundant in CD and UC than healthy controls (2750 vs. 1350 vs. 230 median cfu/biopsy, P = .01). Intracellular E. coli were more prevalent in CD (90%) than UC (47%) or healthy control mucosal biopsies (0%)(P < .001). Extraintestinal pathogenic E. coli virulence factors and AMR genes were identified in E. coli strains from all 3 groups. 2/24 CD strains were adherent and invasive, but there were no unifying pathogenicity determinants that could distinguish most CD strains from UC and healthy control strains, or intracellular isolates from mucosa-associated isolates. Conclusion: Intracellular E. coli are more common in CD than UC or healthy controls. Most intracellular CD E. coli did not have characterising pathogenic features, thus suggesting a greater role for defective mucosal immunity in their ability to gain intracellular access.