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Title: Comparative transcriptomics involving greenbug and water-deficit stress responses in hard-red winter wheat

item REDDY, SRIRAMA - Texas Agrilife Research
item LIU, SHUYU - Texas Agrilife Research
item AKHUNOVA, ALINA - Kansas State University
item Weng, Yiqun
item RUDD, JACKIE - Texas Agrilife Research
item XUE, QINGWU - Texas Agrilife Research
item Payton, Paxton
item Mahan, James

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/15/2012
Publication Date: 1/16/2013
Citation: Reddy, S.K., Liu, S., Akhunova, A., Weng, Y., Rudd, J., Xue, Q., Payton, P.R., Mahan, J.R. 2013. Comparative transcriptomics involving greenbug and water-deficit stress responses in hard-red winter wheat [abstract]. Plant and Animal Genome Conference. Paper No. P0787.

Interpretive Summary:

Technical Abstract: Sessile nature of plants necessitates them to perceive impending cues about biotic and abiotic stresses and alter their growth accordingly by eliciting appropriate defense signals. During the evolutionary progression, plants have developed sophisticated mechanisms to precisely identify the nature of stress and trigger cascade of relevant signals. However, despite abundances of transcriptomic, proteomic, and metabolomic data there is very little information about the parallels or contrasts between biotic and abiotic stress mechanisms at molecular level. In the current study, we have integrated the transcriptomic data generated in response to greenbug (Schizaphis graminum) infestation and water-deficit stress in hard-red winter wheat. The objectives of this study are to identify the patterns in gene expressions that are unique to greenbug and drought stress and that are common to both. We used Affymetrix GeneChip Wheat Genome arrays for two independent transcriptomic studies and GeneSpring v.12 for data analysis. Following normalization, a multivariate ANOVA and Benjamini Hochberg false discovery rate (P<0.01) resulted in 21612 and 26833 differentially regulated transcripts for water-deficit stress and greenbug feeding, respectively. A joint analysis on transcripts with more than two fold change (5896 and 9301 for drought and greenbug respectively) categorized genes unique to greenbug feeding (6300), water-deficit stress (2895) and that were common to both (3001). Functional analysis identified the role of hormone, secondary metabolites, stress-signaling, cell wall modification, heat shock protein, and proteolysis related transcripts in mediating defense responses.