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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Poultry Microbiological Safety and Processing Research Unit » Research » Publications at this Location » Publication #288670

Title: Efficacy of chemical sprays to eliminate inoculated Salmonella from defeathered broiler breast skin

item Cox, Nelson - Nac
item Buhr, Richard - Jeff
item Berrang, Mark
item Bourassa, Dianna
item Rigsby, Luanne
item Oakley, Brian
item RUSSELL, S - University Of Georgia

Submitted to: International Poultry Scientific Forum
Publication Type: Abstract Only
Publication Acceptance Date: 1/29/2013
Publication Date: 1/29/2013
Citation: Cox Jr, N.A., Buhr, R.J., Berrang, M.E., Bourassa, D.V., Rigsby, L.L., Oakley, B., Russell, S.M. 2013. Efficacy of chemical sprays to eliminate inoculated Salmonella from defeathered broiler breast skin [abstract]. International Poultry Scientific Forum. January 29-31,2013. Atlanta, Georgia. P.55.

Interpretive Summary:

Technical Abstract: Contamination of poultry with Salmonella is a food safety concern. Because Salmonella-free broilers cannot always be produced on the farm, antimicrobials are used in the processing plant. The objective of this study was to look at the efficacy of several chemicals to eliminate 104 cells of a marker S. Typhimurium inoculated onto the breast skin of seven week old broiler carcasses. The breast skin on each side of 35 defeathered broilers (70 skin samples) was inoculated with 0.1 mL of a 105 S. Typhimurium. After drying for 2 min, the following hand spray treatments were applied to the breast area of the hanging carcasses (21 mL/carcass): (1) no spray, (2) water spray, (3) 1.4% H2O2, (4) 2.5 % Ox-B (5) 4% Ox-B, (6) 6% Ox-B, (7) 50 ppm chlorine. Ox-B is a stable complex of reactive oxygen species comprised of H2O2 and Cl2. After treatment, carcasses remained hanging/dripping for 2 min and then skin samples (5cm2) were aseptically removed, placed in an 80 mL stomacher bag with 30 mL of 1% buffered peptone and stomached for 1 min. Bags were placed at 37oC for 24h and then samples streaked onto BGSulfa agar plates containing 200 ppm nalidixic acid. Plates were incubated at 37oC for 24h. The number of positive skin samples/number sampled were 10/10, 9/10, 8/10, 0/10, 0/10, 1/10, and 7/10 for treatments 1-7 (listed above) respectively. Ox-B biocide eliminated inoculated Salmonella on all but one skin sample compared to the results for H2O2 (8/10) and 50 ppm Cl2 (7/10). Chlorine and H2O2 both have been shown to be very effective for eliminating Salmonella in previous studies using whole carcass rinse sampling. This research demonstrates that the combination chemical Ox-B can eliminate Salmonella on the skin of defeathered broiler carcasses when applied as a spray prior to immersion chilling. Key words: breast skin, chemical spray, Salmonella