Skip to main content
ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #288659
Title: Complete genome sequence analysis of an American isolate of Grapevine virus E

Author
item ALABI, O - Washington State University
item POOJARI, S - Washington State University
item Sarver, Kara
item Martin, Robert
item RAYPATI, N - Washington State University

Submitted to: Virus Genes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/24/2012
Publication Date: 4/4/2013
Citation: Alabi, O.J., Poojari, S., Sarver, K.E., Martin, R.R., Raypati, N.A. 2013. Complete genome sequence analysis of an American isolate of Grapevine virus E. Virus Genes. 46(3):563-6.

Interpretive Summary: This is the first report of Grapevine virus E in the Pacific Northwest. The virus was completely sequenced, the first sequence of GVE in grapevine from the United States, and found to be very similar to the isolates sequenced from Japan and South Africa. Sequence analysis of GVE isolate from Washington indicated that it is closely related to SA94 and TvP15 isolates from South Africa and Japan, respectively, and distantly related to TvAQ7 from Japan. Although the economic impact of GVE is yet to be realized, information on GVE variability will help in the development of better diagnostic tools to detect all strains of the virus in clean plant programs and provide virus-tested planting materials for wine grape growers in the Pacific Northwest region.

Technical Abstract: The complete genome sequence of an isolate of Grapevine virus E (GVE) collected from a red-berried wine grape cultivar (Cabernet Sauvignon) in Washington State was determined. The 7,568 nt long genome of GVE is similar in size and sequence identity with a GVE isolate from a wine grape cv. Shiraz from South Africa than with an isolate from 'Aki Queen' grape from Japan. Similar to GVE isolates from South Africa and Japan, the Washington isolate encodes five open reading frames (ORFs), and the overall genome organization is similar among these isolates. In addition to AlkB domain, a DExD domain, belonging to the DEAD-like helicases superfamily, was present upstream of the helicase domain in the replicase ORF of the virus.