Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 11/20/2012
Publication Date: 1/12/2013
Citation: Fleetwood, K.M., McDaneld, T.G., Kuehn, L.A., Keele, J.W. 2013. Chromosomal regions impacting pregnancy status in Braford, Brangus and Simbrah cattle [Abstract]. Plant & Animal Genome XXI Conference, July 12-16, 2013, San Diego, CA. Paper No. P0570.
Technical Abstract: We aimed to validate SNP associated with reproductive success in cattle. Cattle were individually genotyped for 73 SNP which were previously identified as significantly associated with reproductive success based on a previous study utilizing DNA pooling. Pregnancy status data were recorded from 2007 to 2008 on 1,842 heifers for their first two breeding seasons from a cooperating ranch in Central Florida consisting of 578 Brangus, 650 Braford and 614 Simbrah. Eighteen pools were created based on pregnancy status with approximately 100 animals per pool (range 55-126 animals). Pools were genotyped by GeneSeek on the Illumina BovineHD beadchip assay. The majority (62) of the 73 significant SNPs were located on chromosome 5. We validated SNP on individuals in the same population using the Sequenom MassARRAY system. Data were analyzed using a generalized linear model in R with pregnancy status as the dependent variable and breed and genotype as fixed effects. Sixty-four (88%) of the 73 SNPs were significant (P < 0.05). Forty-nine SNPs were significant for only additive, 11 SNPs were significant for both additive and dominance, 2 SNPs significant for dominance only, and 3 SNPs were not significant for either dominance or additive effects. Sixty-eight (93%) of the 73 SNPs were significant by individual genotyping and pooling. Fifty-nine (87%) of the 68 significant SNP had consistent effects with the same sign for individual genotyping and pooling. In conclusion, regions on chromosomes 5, 9, 12, 16 and 29 were identified to harbor genetic variation affecting reproductive efficiency in Bos indicus influenced cattle.