Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: 11/6/2012
Publication Date: 1/12/2013
Citation: Casas, E., Hessman, B.E., Keele, J.W., Ridpath, J.F. 2013. A genome-wide association study for the incidence of persistent bovine viral diarrhea virus infection in cattle. Plant and Animal Genome. Proceedings of the Plant Animal Genome XXI. San Diego, CA. p.0560. Interpretive Summary:
Technical Abstract: Bovine viral diarrhea virus (BVDV) is diverse group of viruses causing disease in ruminants. It is controlled with vaccination, biosecurity, and removal of persistently infected animals. The objective was to determine whether genomic regions harbored single nucleotide polymorphisms (SNP) associated with presence or absence of persistent BVDV infections in cattle. A genome-wide association approach based on 777,000 SNP markers was used. Samples of animals identified as positive for the presence of BVDV in skin samples (n= 800), and animals negative for the presence of the BVDV in skin samples (n= 800) were included in the study. DNA samples were incorporated in 16 pools (100 animals per pool) to include the largest number of animals with minimal loss of statistical power. An SNP was declared significant when the p-value exceeded genome-wide value of P < 6.5 x 10-8. A total of 1156 SNP were significantly associated with incidence of BVDV. Of these, 474 SNP mapped to known genes in the bovine genome. The most significant SNP resided in genes on chromosomes 1 (COX17), 3 (EIF2C4 and LRRFIP1), 4 (CALD1, U3SNRP, and COPG2), 6 (MGC127695), 8 (ADAM28), 11 (NRXN1), 13 (TGM3 and DIP2C), 14 (TRMT12 and HDLBP), 16 (PDPN), 17 (CIT), 18 (ZNF599), 19 (SAP30BP), 24 (CABYR and DTNA), X (SHROOM2), and Mitochondrial (ATP6, COX1, CYTB, ND3, ND4, ND4L and ND5). These genes are mostly associated with energy metabolism and RNA transcription. These results contribute to identify pathways associated with the establishment and maintenance of persistent BVDV infection in cattle.