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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Soybean Genomics & Improvement Laboratory » Research » Publications at this Location » Publication #287239

Title: Dynamic proteome analysis of roots of soybean compatible and incompatible to Heterodera glycines

Author
item CHEN, XI - Nanjing Agricultural University
item Garrett, Wesley
item Macdonald, Margaret - Peggy
item Khan, Farooq
item Matthews, Benjamin - Ben
item Natarajan, Savithiry - Savi

Submitted to: Current Protemics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/10/2013
Publication Date: 1/10/2013
Citation: Chen, X., Garrett, W.M., Macdonald, M.H., Khan, F.H., Matthews, B.F., Natarajan, S.S. 2013. Dynamic proteome analysis of roots of soybean compatible and incompatible to Heterodera glycines. Current Protemics. 10:278-291.

Interpretive Summary: The soybean cyst nematode (SCN) is the most devastating pest of soybean in the United States, causing an estimated $2 billion in annual losses. To improve soybean yields by increasing the level of plant resistance to targeted pests, it is important to understand the role of soybean proteins during the interaction of soybean with the SCN. To accomplish this task, we are using a “proteomics” approach in which SCN infected soybean root proteins were separated, identified and quantified using a device called a mass spectrometer. We found differentially expressed proteins between susceptible and resistant responses to SCN. This study will be useful to scientists who wish to develop nematode resistant soybeans.

Technical Abstract: To gain new insights into the mechanism of soybean (Glycine max) interaction with the soybean cyst nematode (Heterodera glycines), we compared protein expression profiles of soybean roots infected by the soybean cyst nematode. Proteins were extracted from roots of 3 and 8 days post-inoculation (dpi) by soybean cyst nematode Heterodera glycines and separated by 2-DE. A total of 42 spots were found to be differentially expressed between the susceptible and resistant responses. After in-gel digestion with trypsin, 39 spots representing 33 proteins were subsequently identified by LC-MS/MS. The proteins showing 1.5-fold changes in intensities are related to biochemical processes that may be differentially altered at various time points after soybean cyst nematode challenge. The identified proteins belong to the categories of metabolism, energy, cell growth and division, transcription, protein synthesis, protein destination and storage, signal transduction, disease/defense, and secondary metabolism. Taken together, our study provides important information on the use of proteomic methods for studying protein regulation during soybean-soybean cyst nematode interactions.