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United States Department of Agriculture

Agricultural Research Service

Research Project: DIETARY MODULATION OF OBESITY-RELATED CANCER BY SELENIUM

Location: Dietary Prevention of Obesity-related Disease Research

Title: Selenium and selenoprotein deficiencies induce widespread pyogranuloma formation in mice, while high levels of dietary selenium decrease liver tumor size driven by TGFa )

Author
item Moustafa, Mohamed
item Carlson, Bradley
item Anver, Miriam
item Bobe, Gerd
item Zhong, Nianxin
item Ward, Jerrold
item Parella, Christine
item Hoffmann, Victoria
item Rogers, Keith
item Combs, Gerald
item Schweizer, Ulrich
item Merlino, Glenn
item Gladyshev, Vadim
item Hatfield, Dolph

Submitted to: PLoS One
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/21/2013
Publication Date: 2/27/2013
Publication URL: http://handle.nal.usda.gov/10113/56706
Citation: Moustafa, M., Carlson, B.A., Anver, M.R., Bobe, G., Zhong, N., Ward, J.M., Parella, C.M., Hoffmann, V.J., Rogers, K., Combs, G.F., Schweizer, U., Merlino, G.T., Gladyshev, V.N., Hatfield, D.L. 2013. Selenium and selenoprotein deficiencies induce widespread pyogranuloma formation in mice, while high levels of dietary selenium decrease liver tumor size driven by TGFa . PLoS One. 8(2): e57389. doi:10.1371/journal.pone.0057389.

Interpretive Summary: This paper addressed the fact that changes in dietary selenium and selenoprotein status can influence both anti- and pro-cancer pathways, making the outcome of interventions different from one study to another. It reports the results of an experiment in which several levels of dietary selenium were fed to mice carrying a either or both of two mutant transgenes: one for selenocysteine tRNA and another that predisposed them to liver cancer. This allowed us to alter selenoprotein expression in individuals fed adequate dietary levels of selenium. We found that mice deprive of selenium developed early morbidity and mortality and showed a characteristic neurologically lethal phenotype. Pathological examination showed inflammatory changes in liver, lungs, heart, spleen, small and large intestine, and mesenteric lymph nodes. Neither dietary selenium nor selenium status, separately or in combination, influenced the development of liver cancer in predisposed mice.

Technical Abstract: Changes in dietary selenium and selenoprotein status may influence both anti- and pro-cancer pathways, making the outcome of interventions different from one study to another. To characterize such outcomes in a defined setting, we undertook a controlled hepatocarcinogenesis study involving varying levels of dietary selenium and altered selenoprotein status using mice carrying a mutant (A37G) selenocysteine tRNA transgene (TrsptG37) and/or TGFa. The use of TrsptG37 allowed us to alter selenoprotein expression in a selenoprotein and tissue specific manner and, at sufficient dietary selenium, separate the effect of diet and selenoprotein status, whereas TGFa was employed as the driver of hepatocarcinogenesis. We subjected these mice to diets deficient in selenium (0.02 ppm selenium) or supplemented with 0.1, 0.4 or 2.25 ppm selenium as selenite or 30 ppm triphenylselenonium chloride (TPSC), a non-metabolized selenium compound. TrsptG37 transgenic and TGFa/TrsptG37 bi-transgenic mice maintained on selenium-deficient or TPSC diets developed early morbidity and mortality prior to tumor formation, and showed a characteristic neurologically lethal phenotype. Pathological analyses revealed widespread disseminated pyogranulomatous inflammation, wherein pyrogranulomas occurred in liver, lungs, heart, spleen, small and large intestine, and mesenteric lymph nodes in the TrsptG37 transgenic and TGFa/TrsptG37 bi-transgenic mice fed the selenium-deficient or TPSC diets. The incidence of hepatocarcinogenesis was significantly increased in mice carrying the TGFa transgene; however, neither dietary selenium nor selenium status, separately or in combination, influenced hepatocarcinogenesis in these animal models. Thus, dietary selenium and selenoprotein status do not affect hepatocarcinogenesis driven by TGFa, but their deficiency leads to widespread pyogranuloma formation.

Last Modified: 8/24/2016
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