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United States Department of Agriculture

Agricultural Research Service

Research Project: Ecology, Genomics, and Management of Stored Product Insects

Location: Stored Product Insect and Engineering Research

Title: A digestive prolyl carboxypeptidase in Tenebrio molitor larvae

item Goptar, Irina
item Shagin, Dmitry
item Shagina, Irina
item Mudrik, Elena
item Smirnova, Yulia
item Zhuzhikov, Dmitry
item Belozersky, Mikhail
item Dunaevsky, Yakov
item Oppert, Brenda
item Filippova, Irina Yu.
item Elpidina, Elena

Submitted to: Journal of Insect Biochemistry and Molecular Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/28/2013
Publication Date: 4/23/2013
Publication URL:
Citation: Goptar, I.A., Shagin, D.A., Shagina, I.A., Mudrik, E.S., Smirnova, Y.A., Zhuzhikov, D.P., Belozersky, M.A., Dunaevsky, Y.E., Oppert, B.S., Filippova, I., Elpidina, E.N. 2013. A digestive prolyl carboxypeptidase in Tenebrio molitor larvae. Journal of Insect Biochemistry and Molecular Biology. 43(6): 501-509.

Interpretive Summary: We are studying enzymes in insects to determine their function and to determine whether we can interfere with the way that they operate to develop new insect control methods. We examined the function of one enzyme, prolyl carboxypeptidase (PRCP), in the yellow mealworm, a pest of stored products. We discovered that PRCP aids in the digestion of proteins in cereals. Our data indicate that the enzyme may be a target for the development of new control methods to prevent damage to stored grains and products.

Technical Abstract: Prolyl carboxypeptidase (PRCP) was purified from the anterior midgut of larvae of a stored product pest, Tenebrio molitor. The cDNA of PRCP was cloned, and the predicted protein was identical to the proteomic sequences of the purified enzyme. The substrate specificity of the enzyme was studied, and kinetic parameters of the substrate hydrolysis were calculated. T. molitor enzyme participates in the hydrolysis of the insect’s major dietary proteins, gliadins, and is the first digestive PRCP to be described.

Last Modified: 10/16/2017
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