|Yokomi, Raymond - Ray|
Submitted to: Frontiers in Microbiology
Publication Type: Abstract only
Publication Acceptance Date: 10/20/2012
Publication Date: 12/10/2013
Citation: Wang, J., Orhan, B., Kwon, S., Rucker, T., Yokomi, R.K., Lee, R.F., Folimonova, S.Y., Greer, G., Krueger, R., Bash, J., Vidalakis, G. 2013. Past and future of a century old Citrus Tristeza virus collection: A California citrus germplasm tale. Frontiers in Microbiology. Available:http://www.dx.doi.org/10.3389/fmicb.2013.00366. Interpretive Summary:
Technical Abstract: The California Citrus Clonal Protection Program (CCPP) provides a mechanism for introduction and distribution of pathogen-free citrus varieties to California for use in research, variety improvement, or commercial production. Citrus tristeza virus (CTV) is a serious citrus pathogen worldwide. The presence of CTV in introduced citrus germplasm as well as from dooryard and field trees in California has been determined and documented for decades at the CCPP. Representative CTV strains or isolates have been maintained in planta in the CCPP quarantine facilities in germplasm dating back to 1907. Genotypes of 48 CTV isolates from this collection were characterized by reverse transcription-polymerase chain reaction amplification with multiple molecular markers targeting different CTV genome regions and by full-length sequencing of the major coat protein gene. These results were compared with symptom expression in biological indicator plants. T30-, T36- and VT-like genotypes in single or mixed infections were identified but no clear association was found between symptom severity and genotype of the CTV isolate. This study provides a valuable historical view of CTV genetic diversity in California under the prism of a germplasm program that created a CTV exclusion bottleneck. These data will be useful for research efforts on development of CTV management strategies such as cross-protection and further genetic characterization of CTV in California.