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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #285494

Research Project: Enhancement of Hard Spring Wheat, Durum, and Oat Quality

Location: Cereal Crops Research

Title: Variability in Arabinoxylan, Xylanase activity and Xylanase inhibitor levels in hard spring wheat

Author
item Mendis, Mihiri - North Dakota State University
item Ohm, Jae-bom
item Delcour, Jan - Katholieke University
item Gebruers, Kurt - Katholieke University
item Meinhardt, Steven - North Dakota State University
item Simsek, Senay - North Dakota State University

Submitted to: Cereal Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/25/2013
Publication Date: 6/1/2013
Publication URL: http://handle.nal.usda.gov/10113/58102
Citation: Mendis, M., Ohm, J., Delcour, J., Gebruers, K., Meinhardt, S., Simsek, S. 2013. Variability in Arabinoxylan, Xylanase activity and Xylanase inhibitor levels in hard spring wheat. Cereal Chemistry. 90:240–248.

Interpretive Summary: Arabinoxylans (AX) are one of the primary components of dietary fibers in wheat. Xylanase is the name of enzymes which degrade arabinoxylans, and xylanase inhibitors are molecules that decrease xylanase activity. The AX, xylanase, and xylanase inhibitors have an important role in many cereal food processing applications. The variations in AX, apparent xylanase and apparent xylanase inhibition activities were investigated for six hard red and six hard white spring wheat genotypes grown at three locations in this research. Individual wheat genotypes showed largely different responses for total AX contents according to growing locations. For bran xylanase activity, genotypes also showed unstable responses to growing locations. Two types of xylanase inhibitors, Triticum aestivum xylanase inhibitor (TAXI) and xylanase inhibiting protein (XIP), were investigated in this research. Hard spring wheat genotypes showed more consistent variability in TAXI activity than XIP across growing locations. These results indicate that TAXI might be a stable parameter in segregating wheat genotypes with varying xylanase activity.

Technical Abstract: Arabinoxylans (AX), xylanase, and xylanase inhibitors have an important role in many cereal food processing applications. The effect of genotype (G), growing location (L), and their interaction (G*L) on AX, apparent xylanase and apparent xylanase inhibition activities of Triticum aestivum xylanase inhibitor (TAXI) and xylanase inhibiting protein (XIP) was investigated for six hard red and six hard white spring wheat genotypes grown at three locations. Variability in total AX content was largely dependent on G*L. For bran xylanase activity, 25% of the variability could be attributed to G*L interaction. Genotype contributed 72% to the variability in TAXI activity and 39 % in XIP. These results indicate that TAXI might be a stable parameter in segregating wheat genotypes with varying xylanase activity.