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ARS Home » Northeast Area » Boston, Massachusetts » Jean Mayer Human Nutrition Research Center On Aging » Research » Publications at this Location » Publication #284762

Research Project: DIETARY CAROTENOIDS, RETINOIDS, AND BIOACTIVATES ON HEALTHY AGING

Location: Jean Mayer Human Nutrition Research Center On Aging

Title: Pharmacological dose of alpha-tocopherol induces cardiotoxicity in Wistar rats determined by echocardiography and histology

Author
item Nascimento, Maria Carolina - Sao Paulo State University (UNESP)
item Matsubara, Beatriz - Sao Paulo State University (UNESP)
item Matsubara, Luiz - Sao Paulo State University (UNESP)
item Correa, Camila - Sao Paulo State University (UNESP)
item Pereira, Elenize - Sao Paulo State University (UNESP)
item Moreira, Priscila - Sao Paulo State University (UNESP)
item Carvalho, Flavio - Sao Paulo State University (UNESP)
item Burini, Caio - Sao Paulo State University (UNESP)
item Padovani, Carlos - Sao Paulo State University (UNESP)
item Yeum, Kyung-jin - Jean Mayer Human Nutrition Research Center On Aging At Tufts University
item Ferreira, Ana Lucia - Sao Paulo State University (UNESP)

Submitted to: Human and Experimental Toxicology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/11/2011
Publication Date: 10/20/2011
Citation: Nascimento, M., Matsubara, B.B., Matsubara, L.S., Correa, C.R., Pereira, E.J., Moreira, P.L., Carvalho, F.A., Burini, C.H., Padovani, C.R., Yeum, K., Ferreira, A.A. 2011. Pharmacological dose of alpha-tocopherol induces cardiotoxicity in Wistar rats determined by echocardiography and histology. Human and Experimental Toxicology. 30(10):1540-1548.

Interpretive Summary: The effect of a supraphysiological dose (greater than normally present in the body) of vitamin E on heart health was studied in Wistar rats. Animals were randomly assigned to either the control or the vitamin E group. Animals received corn oil or vitamin E dissolved in corn oil for a 7-week period. Rats then underwent an exam using a type of ultrasound test (i.e. echocardiogram) to compare heart muscle tissue anatomy at the end of the study period. Vitamin E levels were also assessed. As compared to the control group, the vitamin E-supplemented group showed substantially lower body weight and total heart weight; heart muscle tissue anatomy displayed functional disabilities. The vitamin E uptake in target tissue was confirmed by determination of vitamin E concentrations in heart tissue. The current study indicates that supplementation with supraphysiological levels of vitamin E can induce toxicity in the heart of healthy rats.

Technical Abstract: The effect of pharmacological dose of alpha-tocopherol on heart health was determined in Wistar rats. Animals were randomly assigned to either C (control, n = 11) or E (alpha-tocopherol, n = 11) group. Animals received corn oil (C) or alpha-tocopherol dissolved in corn oil (250 mg alpha-tocopherol/[kg body wt/day]) (E) by gavage for a 7-week period. Rats underwent echocardiogram and were analyzed for cardiomyocyte histology and cardiac alpha-tocopherol absorption at the end of the study period. As compared to the C group, alpha-tocopherol-supplemented group showed significantly (p < 0.05) lower body weight (E, 412.8 g vs C, 480.3 g) and total cardiac weight (E, 0.94 g vs C, 1.08 g); cardiomyocyte histological impairment; smaller left ventricle (LV) (LV end-diastolic diameter (E, 7.22 mm vs C, 7.37 mm), lower LV systolic [left ventricle fractional shortening (E, 47.6% vs C, 53.6%) and ejection fraction ratio (E, 85.4 vs C, 89.9)] and diastolic [early peak velocities of diastolic transmitral flow (E, 64.6 cm/sec vs C, 75.1 cm/sec)] function. The alpha-tocopherol uptake in target tissue was confirmed by determination of alpha-tocopherol concentration medians in cardiac tissue (E, 109.91 nmol/kg vs C, 52.09 nmol/kg). The current study indicates that pharmacological dose of alpha-tocopherol supplementation can induce cardiotoxicity in healthy rats.