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ARS Home » Southeast Area » Dawson, Georgia » National Peanut Research Laboratory » Research » Publications at this Location » Publication #284727

Title: Microsatellite markers in plant pathogenic fungi

item Arias De Ares, Renee
item Ray, Jeffery - Jeff
item Mengistu, Alemu
item Glynn, Neil
item Scheffler, Brian

Submitted to: Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/5/2012
Publication Date: 3/5/2012
Citation: Arias, R.S., Ray, J.D., Mengistu, A., Glynn, N.C., Scheffler, B.E. 2012. Microsatellite markers in plant pathogenic fungi. Meeting Proceedings.

Interpretive Summary: none required.

Technical Abstract: Knowing the genetic diversity of plant pathogenic fungi is essential in the management of crops and disease. The genetic variability of fungal pathogens can be evaluated using molecular markers, among which, microsatellites are a relatively inexpensive source of information. We have developed an efficient method to isolate microsatellites of organisms from which no genome sequence is available. We have also adapted this method to high throughput sequencing in order to generate a large number of markers for genetic mapping. We have shown the use of these markers in developing hypotheses to explain host-pathogen interactions. We will discuss the development and use of microsatellite markers for three plant pathogenic fungi, Macrophomina phaseolina, Cercospora sojina and Puccinia kuehnii.