Location: Arthropod-borne Animal Diseases ResearchTitle: Design of a shear-thinning recoverable peptide hydrogel from native sequences and application for influenza H1N1 vaccine adjuvant
|Huang, Hongzhou - Kansas State University|
|Shi, Jishu - Kansas State University|
|Laskin, Julia - Pacific Northwest National Laboratory|
|Liu, Ziyan - Kansas State University|
|Mcvey, D Scott - Scott|
|Sun, Xiuzhi - Kansas State University|
Submitted to: Soft Matters
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/25/2011
Publication Date: 7/21/2011
Publication URL: http://pubs.rsc.org/en/content/articlepdf/2011/sm/c1sm05157a
Citation: Huang, H., Shi, J., Laskin, J., Liu, Z., Mcvey, D.S., Sun, X.S. 2011. Design of a shear-thinning recoverable peptide hydrogel from native sequences and application for influenza H1N1 vaccine adjuvant. Soft Matters.
Interpretive Summary: A self-assembling peptide was designed and synthesized by rationally combining two native sequences from an elastic segment of spider silk and a trans-membrane segment of human muscle L-type calcium channel. The peptide forms two distinct hydrogels in Ca2+ solution and acidic pH conditions. The shear-thinning, rapid-strength-recovering Ca2+ hydrogel has great potential for drug delivery and tissue engineering applications, for example, as an H1N1 influenza vaccine adjuvant.
Technical Abstract: Peptide hydrogels are considered injectable materials for drug delivery and tissue engineering applications. Most published hydrogel-forming sequences contain either alternating-charged and noncharged residues or amphiphilic blocks. Here, we report a self-assembling peptide, h9e (FLIVIGSIIGPGGDGPGGD), designed by rationally combining two native sequences from an elastic segment of spider silk and a trans-membrane segment of human muscle L-type calcium channel. The turning segment GSII of h9e promoted hydrogel formation in both Ca2+ solution and acidic pH conditions at water content greater than 99.5%. Although h9e Ca2+ hydrogel and h9e acidic hydrogel have the same sequence, they have distinct physical properties. The shear-thinning, rapid-strength-recovering h9e Ca2+ hydrogel was used as an H1N1 influenza vaccine adjuvant. The h9e adjuvant was biologically safe and improved immune response by _70% compared with an oil-based commercial adjuvant.