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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 5/7/2012 Publication Date: N/A Citation: N/A Interpretive Summary: n/a Technical Abstract: The status and preliminary findings for full genome sequencing of three species of microsporidia with mosquitoes as type hosts will be presented. Vavraia culicis, the type species of the genus Vavraia, was originally described from Culex pipiens. Type material was not available and therefore Vavraia culicis floridensis isolated from Aedes albopictus in Florida was used for sequencing. V. culicis has a broad mosquito host range, is infectious for several species of Lepidoptera and characterized by having only uninucleate stages and produces uninucleate spores in multisporous sporophorous vesicles. Anncaliia (=Nosema) algerae, originally described from Anopheles stephensi, has one of the broadest host ranges of any species of microsporidia including many invertebrate and vertebrate hosts. A. algerae is characterized by binucleate (diplokaryotic) stages and is disporous producing individual diplokaryotic spores without a sporophorous vesicle. Edhazardia aedis is the type species for the genus and has a limited host range in mosquitoes and can only complete its life cycle in Ae.aegypti. E. aedis is polymorphic, producing 4 distinctive spore types. It is transmitted both horizontally and vertically and requires 2 generations of the mosquito host to complete the life cycle. Genome sequencing for E. aedis and V. culicis floridensis are completed. V. culicis floridensis has a genome size of approximately 6.1Mb while E. aedis is significantly larger at approximately 51Mb. A. algerae sequencing is underway. Preliminary genome features will be presented. |
