Submitted to: Journal of Proteomics
Publication Type: Peer reviewed journal
Publication Acceptance Date: 7/1/2011
Publication Date: 11/18/2011
Citation: Francischetti, I.M., Anderson, J.M., Manoukis, N., Pham, V.M., Ribeiro, J.M. 2011. An insight into the sialotranscriptome and proteome of the coarse bontlegged tick, Hyalomma marginatum rufipes. Journal of Proteomics. 2892-2908. Interpretive Summary: Ticks are mites specialized in acquiring blood from vertebrates as their sole source of food and are important disease vectors to humans and animals. Among the specializations required for this peculiar diet, ticks evolved a sophisticated salivary potion that can disarm their host's hemostasis, inflammation, and immune reactions. Previous transcriptome analysis of tick salivary proteins has revealed many new protein families indicative of fast evolution, possibly due to host immune pressure. The hard ticks (family Ixodidae) are further divided into two basal groups, of hich the Metastriata have 11 genera. While salivary transcriptomes have been described for some of these genera, no tick of the genes Hyalomma has been studied so far.
Technical Abstract: The analysis of 2,084 expressed sequemce tags (EST) from a salivary gland cDNA library lead to the assembly of 1,167 clusters of sequences, allowing identification of 255 contigs and singletons associated with putative salivary secreted componenets, from which 56 coding sequences (CDS) were extracted, annotated, and deposited into GenBank. Additionally, 41 CDS associated with housekeeping functions were also extracted. The data are organized into hyperlinked spreadsheets that can be obtained from the Additional files 1 and 2 available at http://exon.niaid.nih.gov/transcriptome/Hmrufipes/Hmr-S1.xls and http://exon.niaid.nih.gov/trancriptome/Hmrufipes/Hmr-S2.xls, respectively. Several protein families previously found in tick salivary transcriptomes were identified such as the Kunitz, basil tail, madanin, lipocalin, glycine-rich, mucins, immunity-related, and 8.9-kDa family, as well as protein families previously found only in Dermacentor ticks, such as the 9-kDa family. Most of these proteins have no known function. Many orphan proteins were found that have been no match to known proteins but have signal peptides indicative of secterion, suggesting these to be Hyalomma-specific proteins. This annotated data set can assist the discovery of new targets for anti-tick vaccines as well as help to identify pharmacologically active proteins.