|FABER, TREVOR - University Of Illinois|
|HOPKINS, ANNE - University Of Illinois|
|FAHEY, GEORGE - University Of Illinois|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/19/2012
Publication Date: 8/23/2012
Citation: Price, N.P., Faber, T.A., Hopkins, A.C., Vermillion, K., Fahey, G.C. 2012. Value-added prebiotic GGMO oligosaccharides from a high-volume molasses by-product of pine fiber board manufacture [abstract]. American Chemical Society.
Technical Abstract: "Prebiotics" are substances that enhance the growth of beneficial bacteria in the gastrointestinal tract of host animals. To be of value, prebiotics must provide a selective nutrient source for desirable gut bacteria, especially Bifidobacterium, while reducing the incidence of undesirable bacteria such as E. coli or Salmonella. It is necessary to ascertain the composition and structure of prebiotic oligosaccharides so as to predict these potential beneficial properties. Current markets for animal prebiotics are based on inulin, fructans (FOS), xylose oligosaccharides (XOS), and gentio-oligosaccharides (GOS), although there is also considerable interest in mannose oligosaccharides (MOS). In this talk I will discuss a new, high-production source (0.9 tons per day) of galactoglucomannan oligosaccharides (GGMO), isolated from a molasses bio-product of pine board manufacture. Here we report the structural composition of the molasses components as determined by a combination of limited hydrolysis, monosaccharide composition and linkage analysis, size exclusion fractionation and MALDI-TOF/MS analysis of component GGM oligosaccharides, and 1D and 2D NMR techniques. The GGMO has a degree of polymerization (DP) from 4 to 13, with the major component being DP 5-8. The structure of these oligosaccharides is a ß-1,4-linked backbone of Man and Glc residues, with occasional alpha-1,6 branching by single galactosyl units.