Page Banner

United States Department of Agriculture

Agricultural Research Service


Location: Mycotoxin Prevention and Applied Microbiology Research

Title: Fungal bis-naphthopyrones as inhibitors of botulinum neurotoxin serotype A)

item Cardellina, John
item Roxas-duncan, Virginia
item Montgomery, Vicki
item Eccard, Vanessa
item Campbell, Yvette
item Hu, Xin
item Khavrutskii, Ilja
item Tawa, Gregory
item Wallqvist, Anders
item Gloer, James
item Phatak, Nisarga
item Hoeller, Ulrich
item Soman, Ashish
item Joshi, Biren
item Hein, Sara
item Wicklow, Donald
item Smith, Leonard

Submitted to: ACS Medicinal Chemistry Letters
Publication Type: Peer reviewed journal
Publication Acceptance Date: 4/2/2012
Publication Date: 4/2/2012
Citation: Cardellina, J., Roxas-Duncan, V., Montgomery, V., Eccard, V., Campbell, Y., Hu, X., Khavrutskii, I., Tawa, G.J., Wallqvist, A., Gloer, J.B., Phatak, N.L., Hoeller, U., Soman, A., Joshi, B.K., Hein, S.M., Wicklow, D.T., Smith, L.A. 2012. Fungal bis-naphthopyrones as inhibitors of botulinum neurotoxin serotype A. ACS Medicinal Chemistry Letters. 3(5):387-391.

Interpretive Summary: Botulinum neurotoxins (BoNT’s), produced by the bacterium Clostridium botulinum are the causative agents of botulism, a neuroparalytic illness. Food borne or inhalational intoxications can result in sustained paralysis that necessitates long term supportive care consisting of intubation and ventilation. BoNTs are also popularly used for cosmetic purposes, and as therapies for a range of medical conditions. Intentional and/or accidental misuse of the toxins has brought about the urgent needs for effective therapeutics of botulism. The research describes the fungal metabolites “bis-naphthopyrones” as a novel class of BoNT inhibitors deserving further investigation. These are the first fungal natural products reported to inhibit botulinum neurotoxin serotype A.

Technical Abstract: An in silico screen of the Molecular Library Small Molecule Repository (MLSMR) of ˜350,000 compounds and confirmatory bioassays led to identification of the fungal natural product chaetochromin A (1) as an inhibitor of botulinum neurotoxin serotype A (BoNT A). Subsequent acquisition and testing of analogs of 1 uncovered two analogs with improved activity, talaroderxines A (2) and B (3). These are the first fungal metabolites reported to exhibit BoNT/A inhibitory activity.

Last Modified: 8/24/2016
Footer Content Back to Top of Page