Submitted to: Midwestern Section of the American Society of Animal Science
Publication Type: Abstract only
Publication Acceptance Date: 3/21/2012
Publication Date: 3/21/2012
Citation: Song, R., Chen, C., Johnston, L., Kerr, B.J., Weber, T.E., Shurson, G. 2012. High sulfur content in dried distillers grains with solubles (DDGS) protects against oxidized lipids in DDGS by increasing sulfur-containing antioxidants in nursery pigs [abstract]. Midwestern Section of the American Society of Animal Science. Journal of Animal Science 90 (Suppl. 2):11. Interpretive Summary:
Technical Abstract: Some sources of DDGS contain relatively high amounts of oxidized lipids produced from PUFA peroxidation during production process, but it is unclear whether these oxidized lipids negatively affect growth performance and metabolic oxidation status in pigs. The objective of this study was to compare the effects of feeding corn-soybean meal (CON) or 30% DDGS diets with 3 levels of vitamin E: none supplemented, NRC level, and 10X NRC level to nursery pigs. The DDGS source used in this study contained the highest thiobarbituric acid reactive substances (TBARS) value, peroxide value (PV), and total S content (5.2 ng/mg oil, 84.1 meq/kg oil, and 0.95%, respectively), compared to average levels of 30 other DDGS sources (1.8 ng/mg oil, 11.5 meq/kg oil, and 0.50%, respectively) and a reference sample of corn (0.2 ng/mg oil, 3.1 meq/kg oil, and 0.01%, respectively). Fifty-four barrows were housed in pens for 8 wk after weaning and transferred to individual metabolism cages for collection of feces, urine, blood, and liver samples. Total S content was two times higher in DDGS diets than CON (0.39 vs. 0.19%). Sulfur absorbed, retained, and apparent total tract digestibility were higher (P < 0.001) in pigs fed DDGS diets compared to CON. Although pigs were fed highly oxidized DDGS in this study, serum TBARS were similar among treatments. Serum vitamin E level increased by feeding DDGS diets compared to CON (2.25 vs. 1.56 µg/mL, P < 0.001). Pigs fed DDGS had higher concentrations of S-containing AAs, particularly methionine (P < 0.001) and taurine (P = 0.002) in fed serum, and a higher level of taurine in fasted serum (P = 0.006) than those fed CON. Liver glutathione reduced concentration was increased in both DDGS (P < 0.001) and vitamin E (P = 0.01) dietary treatments. These results indicate that adding additional vitamin E to diets containing high S DDGS may not be necessary because pigs had an increased level of S-containing antioxidants (taurine, methionine and glutathione) in vivo, which appear to provide adequate protection against oxidized lipids in DDGS.