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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Reproduction Research » Research » Publications at this Location » Publication #275350

Title: Single nucleotide polymorphisms in sheep varying in tolerance to elevated dietary nitrate

Author
item COCKRUM, REBECCA - University Of Wyoming
item Rempel, Lea
item CAMMACK, KRISTI - State Of Wyoming

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/2/2011
Publication Date: 1/14/2012
Citation: Cockrum, R., Rempel, L., Cammack, K.M. 2012. Single nucleotide polymorphisms in sheep varying in tolerance to elevated dietary nitrate [abstract]. Plant and Animal Genome XX Conference Proceedings. Abstract #P0219.

Interpretive Summary:

Technical Abstract: Discovery of single nucleotide polymorphisms (SNPs) may lead to development of marker panels predictive of tolerance to high dietary nitrate (NO3-). The aims of this research were to identify SNPs in Arginiosuccinate Lyase (ASL), determine the relationship of ASL SNP genotypes on NO3- tolerance, and determine relationships between ASL SNPs and genes previously identified as differentially expressed in response to elevated dietary NO3-. Liver tissues were collected from Suffolk ewes identified as highly tolerant (n = 6) or lowly tolerant (n = 6) to an 8 d administration of 300 mg KNO3/kg of BW 3 x daily. Tissues were also collected from untreated control (n = 6) ewes. Hot Star PCR and iPLEX Gold protocols were used for ASL SNP identification and genotyping, and the MIXED procedure of SAS was used to determine SNP relationships on NO3- tolerance and expression of previously identified genes. Nine polymorphic SNPs were identified within the ASL sequence. None of the ASL SNPs were related (P >/= 0.468) to NO3- tolerance. However, eight ASL SNPs were correlated (P