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ARS Home » Southeast Area » Fort Pierce, Florida » U.S. Horticultural Research Laboratory » Subtropical Insects and Horticulture Research » Research » Publications at this Location » Publication #275094

Title: Isothermal amplification of insect DNA

item Dickey, Aaron
item OSBORNE, LANCE - University Of Florida
item Shatters, Robert - Bob
item McKenzie, Cindy

Submitted to: Entomological Society of America Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 9/8/2011
Publication Date: 11/16/2011
Citation: Dickey, A.M., Osborne, L.S., Shatters, R.G., Mckenzie, C.L. 2011. Isothermal amplification of insect DNA [abstract]. Entomological Society of America Annual Meeting. Paper No. D0542.

Interpretive Summary:

Technical Abstract: The loop-mediated isothermal amplification of DNA (LAMP) method can amplify a target DNA sequence at a constant temperature in about 1 hour. LAMP has broad application in agriculture and medicine because of the need for rapid and inexpensive diagnoses. The power of LAMP is being used by researchers and clinicians for pathogen detection and positive identification but agricultural applications have been much more limited. LAMP should be well suited to the identification of economically and medically important insects, particularly for members of cryptic species complexes, which cannot be distinguished morphologically. Here we report the first loop-mediated isothermal amplification of insect DNA using sweetpotato whitefly Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) biotypes B and Q.