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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » ABADRU » Research » Publications at this Location » Publication #274472

Title: Whole genome sequence analysis of field strains of bluetongue virus

item Wilson, William
item Smith, Timothy - Tim

Submitted to: Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/12/2010
Publication Date: 11/11/2010
Citation: Wilson, W.C., Smith, T.P. 2010. Whole genome sequence analysis of field strains of bluetongue virus. Meeting Proceedings. 154.

Interpretive Summary:

Technical Abstract: The Arthropod Borne Animal Diseases Research Unit(ABADRU) has been developing rapid, high sensitive biosenor technology based on gold nanoparticles and Surface Enhanced Raman Scattering (SERS). The “proof-of-concept” for the nucleic acid and immunological based assays was reviewed. A more recent enhancement in this technology was also discusses. The ABADRU has also adapted the published single primer ligation-whole genome amplification protocol that allows the whole bluetongue virus genome to be amplified without prior sequence knowledge and submitted to high-throughput DNA sequencing. Preliminary data was discussed as well as the potential impact on the ability to rapidly perform molecular evolution analyses.