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Title: Nested PCR and RFLP analysis based on the 16S rRNA gene

item DUDUK, B. - Institute Of Pestiides And Environmental Protection
item PALTRINIERI, S. - University Of Bologna
item Lee, Ing Ming
item BERTACCINI, A. - University Of Bologna

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 8/20/2011
Publication Date: 9/21/2012
Citation: Duduk, B., Paltrinieri, S., Lee, I., Bertaccini, A. 2012. Nested PCR and RFLP analysis based on the 16S rRNA gene. In: Dickinson, M., Hodgetts, J., editors. Springer Protocols: Methods in Molecular Biology. New York, NY: Humana Press. p. 159-172.

Interpretive Summary:

Technical Abstract: Current phytoplasma detection and identification method is primarily based on nested PCR followed by restriction fragment length polymorphism analysis and gel electrophoresis. This method can potentially detect and differentiate all phytoplasmas including those previously not described. The present protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels.