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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Publications at this Location » Publication #270704


Location: Plant, Soil and Nutrition Research

Title: Maize HapMap2 identifies extant variation from a genome in flux

item Chia, Jer-ming
item Song, Chi
item Bradbury, Peter
item Costich, Denise
item De Leon, Natalia
item Doebley, John
item Elshire, Robert
item Gaut, Brandon
item Geller, Laura
item Glaubitz, Jeffrey
item Gore, Michael
item Guill, Kate
item Holland, James
item Hufford, Matthew
item Lai, Jinsheng
item Li, Meng
item Liu, Xin
item Lu, Yanli
item Mccombie, Richard
item Nelson, Rebecca
item Poland, Jesse
item Prasanna, Boddupalli
item Phyajarvi, Tanja
item Rong, Tingzhao
item Sekhon, Rajandeep
item Sun, Qi
item Tenaillon, Maud
item Tian, Feng
item Wang, Jun
item Xu, Xun
item Zhang, Zhiwu
item Kaeppler, Shawn
item Ross-ibarra, Jeffrey
item Mcmullen, Michael
item Buckler, Edward - Ed
item Zhang, Genyun
item Xu, Yunbi
item Ware, Doreen

Submitted to: Nature Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/22/2012
Publication Date: 6/3/2012
Citation: Chia, J., Song, C., Bradbury, P., Costich, D., De Leon, N., Doebley, J., Elshire, R., Gaut, B., Geller, L., Glaubitz, J., Gore, M.A., Guill, K., Holland, J., Hufford, M., Lai, J., Li, M., Liu, X., Lu, Y., McCombie, R., Nelson, R., Poland, J.A., Prasanna, B., Phyajarvi, T., Rong, T., Sekhon, R., Sun, Q., Tenaillon, M., Tian, F., Wang, J., Xu, X., Zhang, Z., Kaeppler, S.M., Ross-Ibarra, J., McMullen, M.D., Buckler IV, E.S., Zhang, G., Xu, Y., Ware, D. 2012. Maize HapMap2 identifies extant variation from a genome in flux. Nature Genetics. 40:803-807. DOI: 10.1038/ng.2313.

Interpretive Summary: Maize is the world’s largest production crop in the world, and its diversity has allowed it in a few thousand years to adapt to the tropics, mountains, temperate locations. Using next generation sequencing technology, we characterize this diversity to an unprecedented level in the world’s key breeding lines and its wild relatives. Surprisingly, nearly 90% of the genome is associated with structural variation, and this variation is responsible for a substantial portion of trait variation in maize. Despite this incredible structural variation, there is tremendous unity in key gene content even with the sister genus, which suggests that adaptations (perennialism, frost and drought tolerance, etc.) amongst all of maize’s relatives are likely integratable in maize.

Technical Abstract: The maize genome is the largest, most diverse and complex plant genome sequenced to date. Using high-throughput sequencing to access genetic variation and a population genetics model to score the polymorphisms, we characterize and unite the diversity of the world’s key breeding germplasm, wild relatives, and its sister genus. With the discovery of more than 55M SNP/indel variants, this permits for the first time true genome-wide association studies (GWAS) in a complex plant genome. Analysis of structural variation (SV) suggests 90% of the genome is segregating for SV, and GWAS indicates SVs are highly enriched for functional associations. This survey provides the foundation to unite genetic dissection and breeding of germplasm resources across maize and its relatives.