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Title: Green tea polyphenols benefits body composition and improves bone quality in long-term high-fat diet-induced obese rats

item SHEN, CHWAN-LI - Texas Tech University
item Cao, Jay
item DAGDA, RAUL - Texas Tech University
item CHANJAPLAMMOOTIL, SAMUEL - Texas Tech University
item LU, CHUWANWEN - Texas Tech University
item CHYU, MING-CHIEN - Texas Tech University
item GAO, WEIMIN - Texas Tech University
item WANG, JIA-SHENG - University Of Georgia
item YEH, JAMES - Winthrop University Hospital

Submitted to: Nutrition Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/18/2012
Publication Date: 6/8/2012
Citation: Shen, C., Cao, J.J., Dagda, R., Chanjaplammootil, S., Lu, C., Chyu, M., Gao, W., Wang, J., Yeh, J.K. 2012. Green tea polyphenols benefits body composition and improves bone quality in long-term high-fat diet-induced obese rats. Nutrition Research. 32:448-457.

Interpretive Summary: Obesity and osteoporosis are two major health disorders in the USA. We investigated the effects of green tea polyphenols (green tea bioactive components) on body composition and bone metabolism in a high-fat diet induced obesity animal model. We found that supplementation green tea polyphenols for 4 months increased fat-free mass, bone mineral content, bone mineral density and bone strength. Our data demonstrate that green tea polyphenols are useful in preventing obesity and are beneficial to bone development.

Technical Abstract: This study investigated the effects of green tea polyphenols (GTP) on body composition and 2 bone properties in obese female rats. Thirty-six 3-month-old SD female rats were fed either a 3 low-fat (LF) diet (n = 12) or a high-fat (HF) diet (n= 24) for 4 months. Animals in the LF diet 4 group continued on an LF diet for additional 4 months, while those in the HF diet group were 5 divided into 2 groups, with 0.5% (wt/vol) or without GTP in drinking water, in addition to an HF 6 diet for another 4 months. Body composition [body weight (BW), fat mass (FM), fat-free mass 7 (FFM), total body water (TBW), intracellular fluid (ICF), and extracellular fluid (ECF)] was 8 measured by bioimpedance spectroscopy. Bone parameters [femoral bone area, mineral content 9 (BMC) and density (BMD)] were measured by dual-energy x-ray absorptiometry. Bone strength, 10 serum proinflammatory cytokines levels, and liver glutathione peroxidase (GPX) activity and 11 protein expression were also determined. After 8 months, compared to the LF diet, the HF diet 12 increased BW, %FM, and ECF/ICF ratio, reduced %FFM, %TBW, GPX activity and protein 13 expression, and bone strength, but had no effect on BMC and BMD in the rats. GTP 14 supplementation for 4 months increased %FFM, BMC, BMD, bone strength, and GPX activity 15 and protein expression, and decreased %FM, the ECF/ICF ratio and cytokines in HF-diet-16 induced obese rats. This study demonstrates that GTP supplementation in drinking water for 4 17 months modifies body composition and enhances bone properties in obese rats through 18 suppressing inflammation and enhancing antioxidant capacity.