Location: Application Technology ResearchTitle: The Dahlia mosaic virus gene VI product N-terminal region is involved in self association) Author
|Krause, Charles - Chuck|
Submitted to: Virus Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/15/2011
Publication Date: 6/15/2011
Publication URL: http://handle.nal.usda.gov/10113/56256
Citation: Raikhey, G., Krause, C.R., Leisner, S. 2011. The Dahlia mosaic virus gene VI product N-terminal region is involved in self association. Virus Research. 159:69-72. DOI:10.1016/j.virusres.2011.04.026. Interpretive Summary: A unique virus pathogen of the popular bedding plant, Dahlia spp, was studied. The study highlights that the protein portion of the pathogen self-sassociated with the host plant in a particular part of the genetic material and begins to explain the intricate functions, the science and impact this economic problem.
Technical Abstract: The genome of the 'oriculture pathogen Dahlia mosaic caulimovirus(DMV) encodes six openreading frames. Generally,caulimovirusgene VIproducts(P6s) are thought to be multifunctional proteins required for viral infection and it is likely that self-association is required for some of these functions. In this study,yeast two-hybrid and maltose binding protein(MBP) pull-down assay sindicated thatf ull-length DMV P6 speci'cally self-associates. Further analyses indicated that only the DMV P6 N-terminal region, consisting of 115 amino acids, interacts with fulllength P6 and with itself. This distinguishes the DMV P6 from its Cauli'ower mosaic virus counterpart, which contains four regions involved in self-association. Thus, our results suggest that each caulimovirus P6 may possess a unique pattern of protein protein interactions. Bioinformatic tools identi'ed a putative nuclear exclusion signal located between amino acid residues 10–20, suggesting another possible function for the P6 N-terminal region..