Location: Sunflower and Plant Biology ResearchTitle: Development of a set of compensating Triticum aestivum-Dasypyrum villosum Robertsonian translocation lines Author
Submitted to: Genome
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/28/2011
Publication Date: 10/1/2011
Citation: Liu, C., Qi, L., Liu, W., Zhao, W., Wilson, J., Friebe, B., Gill, B.S. 2011. Development of a set of compensating Triticum aestivum-Dasypyrum villosum Robertsonian translocation lines. Genome. 54:836-844. Interpretive Summary: Dasypyrum villosum (L.) Candargy is a wild relative of bread wheat native to the Mediterranean. It has broad genetic variability and is a source of agronomically important genes that can be used in wheat improvement. The first step in exploiting this variation is the exchange of certain chromosome arms of D. villosum with the corresponding wheat chromosome arms. So far, only genes conferring resistance to powdery mildew and stem rust from chromosome 6V, resistance to wheat spindle streak mosaic virus from chromosome 4V, and seed quality genes from chromosome 1V have been transferred to wheat. In addition, resistance gene for eyespot is known to be present on chromosome 4V. In this study, we report the development and characterization of six new T. aestivum-D. villosum translocation lines using DNA markers, fluorescence-tagged DNAs, and chromosome staining. All six translocations are fertile, although the level of spike fertility and hundred kernel weight (HKW) varied among the lines. These new lines with chromosome translocations from the wild relative D. villosum will be useful in wheat improvement programs.
Technical Abstract: Dasypyrum villosum (L.) Candargy, a wild relative of bread wheat (Triticum aestivum L.) is the source of agronomically important genes for wheat improvement. The first step in exploiting this variation is the production of compensating Robertsonian translocations (cRobTs) consisting of D. villosum chromosome arms translocated to homoeologous wheat chromosome arms. The cRobTs for D. villosum chromosomes 1V, 4V and 6V have been reported previously. Here we report attempted cRobTs for wheat-D. villosum chromosome combinations 2D/2V#3, 3D/3V#3, 5D/5V#3 and 7D/7V#3. The cRobTs for all D. villosum chromosomes were recovered except for the 2V#3S and 5V#3L arms. As was the case with the 6D/6V combination, no cRobTs involving 2D/2V#3 chromosomes were recovered and instead, cRobT T2BS.2V#3L involving a non-targeted chromosome was recovered. All cRobTs are fertile although the level of spike fertility and hundred kernel weight (HKW) varied among the lines. Among the already reported cRobTs, T6AL.6V#2S carrying the Pm21 gene is deployed in agriculture. Many useful genes have been reported on other cRobTs including resistance to stem rust race UG99 on T6AS.6V#3L. The set of cRobTs involving 12 of the 14 D. villosum chromosomes will be useful in wheat improvement programs.