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Title: Simultaneous determination of alkaloids and flavonoids from aerial parts of Passiflora species and dietary supplements using UPLC-UV-MS and HPTLC

Author
item AVULA, BHARATHI - University Of Mississippi
item WANG, YAN-HONG - University Of Mississippi
item RUMALLA, CHIDANANDA - University Of Mississippi
item SMILLIE, TROY - University Of Mississippi
item KHAN, IKHLAS - University Of Mississippi

Submitted to: Talanta
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/15/2011
Publication Date: 6/15/2012
Citation: Avula, B., Wang, Y., Rumalla, C.S., Smillie, T.J., Khan, I.A. 2012. Simultaneous determination of alkaloids and flavonoids from aerial parts of Passiflora species and dietary supplements using UPLC-UV-MS and HPTLC. Talanta. 7(9):1177-1180.

Interpretive Summary: A newly developed method was found to be useful in identification of plant samples and to ensure the quality of P. incarnata preparations used as herbal supplements. LC-mass spectrometry coupled with electrospray ionization (ESI) method is described for the identification of nine compounds in plant sample and dietary supplements. The fingerprint method developed in this study was found to be simple, fast, cost effective, and can be used for the routine quality control analysis.

Technical Abstract: A rapid UPLC method was developed for the simultaneous analysis of five indole alkaloids (harmalol, harmol, harmane, harmaline and harmine) and four flavonoids (orientin, isoorientin, vitexin, and isovitexin) from the aerial parts of Passiflora incarnata L. (Passifloracea), different species of Passiflora (P. violacea, P. capsularis, P. edulis) and dietary supplements that claim to contain Passiflora. The separation was achieved within 8.0 minutes by using C-18 column material, a water/acetonitrile mobile phase, both containing formic acid using a gradient system and a temperature of 35 degrees Celsius. The method was validated for linearity, repeatability, limits of detection (LOD) and limits of quantification (LOQ). The wavelength used for quantification with the diode array detector was 340 nm for flavonoids and alkaloids. The developed method is simple, economic, fast and especially suitable for quality control analysis of flavonoids and alkaloids in plant samples and dietary supplements. The compounds (including isoschaftoside and schaftoside) in plant samples and commercial products of Passiflora were identified and confirmed by UPLC-MS. A HPTLC method was also developed for the chemical fingerprint analysis of Passiflora samples.