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United States Department of Agriculture

Agricultural Research Service

Research Project: METABOLOMIC AND MICROBIAL PROFILING OF TROPICAL/SUBTROPICAL FRUITS AND SMALL FRUITS FOR QUALITY FACTORS AND MICROBIAL STABILITY

Location: Citrus and Other Subtropical Products Research

Title: Total phenol content of guava fruit and development of an in vitro regeneration protocol amenable to genetic improvement)

Author
item Sitther, Viji
item Harris, Donna
item Dhekney, Sadanand
item Bai, Jinhe
item Baldwin, Elizabeth - Liz
item Yadav, Anand

Submitted to: International Journal Food Safety, Nutrition and Public Health
Publication Type: Peer reviewed journal
Publication Acceptance Date: 9/21/2011
Publication Date: 12/20/2011
Citation: Sitther, V., Harris, D.L., Dhekney, S., Bai, J., Baldwin, E.A., Yadav, A. 2011. Total phenol content of guava fruit and development of an in vitro regeneration protocol amenable to genetic improvement. International Journal Food Safety, Nutrition and Public Health. 4:225-236.

Interpretive Summary: Guava fruit are considred very nutritous due to their high vitamin C, fiber and antioxidants. Seven guava varieties, including pink- and white-fleshed guava were studied for ability to propogate (reproduce) in a Souther US climate. Antioxidant compounds were also measured.

Technical Abstract: Total soluble phenolics in two white (‘Allahabad Safeda’ and ‘Lucknow-49’), two pink (‘Beaumont’ and ‘Gushiken Sweet’), and three red fleshed (‘Ka Hua Kola’, ‘Ruby Supreme’ and ‘Red Fleshed’) guava (Psidium guajava. L.) fruits were assessed using the Folin-Ciocalteu procedure. ‘Allahabad Safeda’ and ‘Ruby Supreme’ contained the highest level of phenols (1967.5 and 1921.25 µg/g gallic acid equivalents. No significant difference in phenolic content was observed between white and red fleshed guava fruits. An in vitro method was developed for clonal propagation of ‘Lucknow-49’ and ‘Gushiken Sweet’ cultivars. Five concentrations of 6-benzylaminopurine (BAP) and kinetin (KIN) plant growth regulators were tested for in vitro regeneration. A maximum of 4.5 shoots per explant was produced on Murashige and Skoog medium supplemented with 8.9 µM BAP + 9.4 µM KIN. These studies indicate that guava, a rich source of natural phenolic antioxidants can be efficiently micropropagated for genetic studies to enhance its nutraceutical value.

Last Modified: 8/24/2016
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