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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Publications at this Location » Publication #264451

Title: Citrus-CTV molecular interactions: What is the host side of the story?

item DODDAPANENI, HARSHA - University Of Iowa
item SAPONARI, MARIA - Institute De Virologia
item GIAMPETRUZZI, ANNALISA - Bari University
item LOCONSOLE, GIULIANA - Bari University
item SALDARELLI, PASQUALE - Institute De Virologia
item Yokomi, Raymond - Ray

Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 3/15/2011
Publication Date: 6/1/2011
Citation: Doddapaneni, H., Saponari, M., Giampetruzzi, A., Loconsole, G., Saldarelli, P., Yokomi, R.K. 2011. Citrus-CTV molecular interactions: What is the host side of the story?. Phytopathology. 101:S43.

Interpretive Summary:

Technical Abstract: Tristeza caused by Citrus tristeza virus (CTV) is an example where cross protection (CP) has been successfully used as a control strategy. However, in most instances, CP has broken down over time. Lack of knowledge of molecular regulatory mechanisms of CP is a major impediment to improvement of sustainability of CP. There are multiple anecdotal reports on virus strains involved in CP, but molecular events in citrus that result in CP are unknown. Deep sequencing (Illumina) analysis of small interfering RNAs (siRNAs) extracted from sour orange (SO) severely affected by seedling yellows (SY) and from the cross protected SO showed atypical accumulation of virus-derived siRNAs mapping to the 3’end of the CTV genome. Contigs from these datasets identified sequences homologous to the 282 Kb Ctv resistance locus of Poncirus trifoliata, providing strong evidence that this region is involved in the siRNA pathways. Similarly, a total of 50 miRNA families had significant sequence presence, of which 8 miRNA families showed differential expression after CTV infection. Analysis of Citrus mRNA targets revealed 433 were common to SO plants showing protection or strong SY symptoms. Other mRNA targets were specific to CP (873) or SO showing severe SY (753). The different mRNA targets defined suggested that different CTV isolates can induce different miRNA-mRNA interactions.