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Title: The Doubled Haploid Facility at Iowa State University

item LUBBERSTEDT, THOMAS - Iowa State University
item FREI, URSULA - Iowa State University
item BOVENMEYER, ELIZABETH - Iowa State University
item VANOUS, ADAM - Iowa State University
item Smelser, Andrew
item Gardner, Candice
item Blanco, Michael

Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/28/2011
Publication Date: 3/17/2011
Citation: Lubberstedt, T., Frei, U.K., Bovenmeyer, E., Vanous, A., Smelser, A.D., Gardner, C.A., Blanco, M.H. 2011. The Doubled Haploid Facility at Iowa State University [abstract]. Maize Genetics Conference Abstracts. Abstract P268.

Interpretive Summary:

Technical Abstract: The development of homozygous lines is an important, but time-consuming process in plant breeding and research. The in vivo induction and subsequent doubling of haploids is an efficient alternative to generate homozygous offspring in two generations. The Doubled Haploid Facility (DHF), established in 2010, provides haploid doubling for maize to ISU scientists, as well as off-campus academic researchers and breeders. The mission of DHF is to provide expertise and service in the production of doubled haploid lines in maize, to improve the technology in order to get higher success rates and lower costs, and to teach and train scientists and students ( Our research objectives are (1) marker development for induction ability, (2) development of inducer lines adapted to different environments, (3) development of technologies for mechanical pre-sorting in haploid selection, (4) introduction of additional selective marker for haploid selection, and (5) finding alternatives to colchicine for chromosome doubling. During our first year, research focused on the comparison of different application methods of colchicine for chromosome doubling.