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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Publications at this Location » Publication #263792
Title: The complete nucleotide sequence and genome organization of a novel carmovirus - Honeysuckle ringspot virus isolated from honeysuckle.

Author
item Gulati Sakhuja, Anju
item RAINS, LINDSAY - California Department Of Food And Agriculture
item TIAN, TONGYAN - California Department Of Food And Agriculture
item Liu, Hsing Yeh

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/20/2011
Publication Date: 5/12/2011
Citation: Gulati Sakhuja, A.N., Rains, L., Tian, T., Liu, H. 2011. The complete nucleotide sequence and genome organization of a novel carmovirus - Honeysuckle ringspot virus isolated from honeysuckle. Archives of Virology. 156:1635-1640.

Interpretive Summary: Honeysuckle (Lonicera sp) is a common perennial wild and ornamental plant that is propagated vegetatively through cuttings. Up to date, only three viruses belonging to different families have been reported infecting honeysuckle including Tobacco streak Ilarvirus , Honeysuckle latent virus, and Honeysuckle yellow vein mosaic virus. In this paper, we report a new carmovirus associated with yellow to purple ring spots on honeysuckle and we tentatively named it, Honeysuckle ringspot virus (HnRSV). Partially purified virions of HnRSV from infected honeysuckle plants were spherical particles of 29 to 31 nm in diameter. Viral-associated double stranded (ds)RNA was isolated from infected honeysuckle leaves. The dsRNA profile consistently revealed one major band of about 4.0 kb and two subgenomic RNAs of approximately 1.7 and 1.3 kb, resembling of carmovirus. The complete genomic RNA of HnRSV has been sequenced. Based on its genome organization, amino acid sequence comparisons and phylogenetic analysis confirms that HnRSV is a definitive member of genus Carmovirus in the family Tombusviridae.

Technical Abstract: A virus associated with yellow to purple ringspot on honeysuckle plants has been detected and tentatively named as Honeysuckle ringspot virus (HnRSV). The complete nucleotide sequence of HnRSV has been determined from infected honeysuckle. The genomic RNA of HnRSV is 3,956 nucleotides in length and is predicted to contain five ORFs. Comparisons of amino acid sequences of the ORFs of HnRSV with members of Tombusviridae show that HnRSV is closely related to members of the genus Carmovirus. Phylogenetic analyses based on the amino acid sequences of RdRp and coat protein and nucleotide sequences of the whole genome revealed that HnRSV forms a subgroup with carmoviruses. . Together, our results support the classification of HnRSV as a new species in the genus Carmovirus, family Tombusviridae.