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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Healthy Processed Foods Research » Research » Publications at this Location » Publication #263503

Research Project: New Sustainable Processing Technologies to Produce Healthy, Value-Added Foods from Specialty Crops and their Co-Products

Location: Healthy Processed Foods Research

Title: Enzymatic hydrolysis of rice protein with papain and antioxidation activity of hydrolysate

item Cao, Hui
item Ma, Haile
item Qu, Wenjuan
item Jia, Junqiang
item Pan, Zhongli - John
item Ding, Qingzhi
item Luo, Lin
item Wang, Zhenbin
item He, Ronghai

Submitted to: Journal of Chinese Cereals and Oils Association
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/2009
Publication Date: 7/1/2009
Citation: Cao, H., Ma, H., Qu, W., Jia, J., Pan, Z., Ding, Q., Luo, L., Wang, Z., He, R. 2009. Enzymatic hydrolysis of rice protein with papain and antioxidation activity of hydrolysate. Journal of Chinese Cereals and Oils Association. 24(7):10-14.

Interpretive Summary: This research studied the effect of processing conditions of hydrolysate from rice protein on its antioxidant activity. The results showed that the hydrolysate has antioxidant capability.

Technical Abstract: The enzymatic hydrolysis technology of rice protein and the antioxidant activity of the hydrolysate were studied. Substrate concentration,enzyme dose,pH value and temperature were selected as factors to optimize the hydrolysis parameters with single—factor and orthogonal tests. Results show the optimum condition for the enzymatic hydrolysis is as follows:substrate concentration 10%,enzyme dose 5%,pH value 6.0,temperature 60',and time 90 min.Under the optimum condition,the soluble solid content of the hydrolysate is 25.6 ms/mE,and the DPPH free radical scavenging rate is 54.5%. Moreover,the result of an experiment shows that the rice protein hydrolysate has very strong scavenging capabilities for DPPH free radical and hydroxyl radical,and their IC50 are 1.738 and 0.238 mg/mL respectively,showing a very strong reducing ability.It is concluded that hydrolysate from rice protein as natural peptide has antioxidant activity.