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United States Department of Agriculture

Agricultural Research Service


Location: Renewable Product Technology Research

Title: Production of 7,10-dihydroxy-8(E)-octadecenoic acid from olive oil by Pseudomonas aeruginosa PR3

item Suh, Min-jung
item Baek, Ka-yeon
item Kim, Beom-soo
item Hou, Ching
item Kim, Hak-ryul

Submitted to: Applied Microbiology and Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/1/2011
Publication Date: 6/30/2011
Publication URL:
Citation: Suh, M., Baek, K., Kim, B., Hou, C.T., Kim, H. 2011. Production of 7,10-dihydroxy-8(E)-octadecenoic acid from olive oil by Pseudomonas aeruginosa PR3. Applied Microbiology and Biotechnology. 89:1721-1727.

Interpretive Summary: Previously, we discovered a new microbial culture Pseudomonas aeruginosa PR3 that produced 7,10-dihydroxy-fatty acid from oleic acid and trihydroxy fatty acids from linoleic acid. Hydroxy fatty acids are useful as starting materials for the synthesis of specialty chemicals, special military nylon, plastisizers, coating materials, and possible as physiologically active agents. However, we need an economical process to produce these value-added hydroxyl fatty acid products ideally direct from natural oils and not from expensive free fatty acids. Earlier we reported that triolein, the triacylglyceride form of oleic acid, was successfully converted to dihydroxy fatty acids product (DOD) by strain PR3 via triolein-induced lipase activity. Now we found that strain PR3 can also efficiently convert natural oil, olive oil directly to produce DOD. Our findings can facilitate the research on hydroxyl fatty acids production from other vegetable oils such as soybean oil by bioprocess and benefit the U.S. farmers.

Technical Abstract: Microbial modification of naturally occurring materials is one of the efficient ways to add new values to them. Hydroxylation of free unsaturated fatty acids by microorganism is a good example of those modifications. Among microbial strains studied for that purpose, a new bacterial isolate Pseudomonas aeruginosa PR3 has been well studied to produce several hydroxy fatty acids from different unsaturated fatty acids. Of those hydroxy fatty acids, 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) was efficiently produced from oleic acid by strain PR3. However, it was highly plausible to use vegetable oil containing oleic acid rather than free oleic acid as a substrate for DOD production by strain PR3. In this study we firstly tried to use olive oil containing high content of oleic acid as a substrate for DOD production. DOD production from olive oil was confirmed by structural determination with GC, TLC, and GC/MS analysis. DOD production yield from olive oil was 53.5%. Several important environmental factors were also tested. Galactose and glutamine were optimal carbon and nitrogen sources and magnesium ion was critically required for DOD production from olive oil. Results from this study demonstrated that natural vegetable oils containing oleic acid could be used as efficient substrate for the production of DOD by strain PR3.

Last Modified: 06/24/2017
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