|Liu, Hsing Yeh|
|Gulati Sakhuja, Anju|
Submitted to: American Society of Sugarbeet Technologists
Publication Type: Proceedings
Publication Acceptance Date: 5/1/2011
Publication Date: 6/1/2011
Citation: Liu, H., Gulati Sakhuja, A.N. 2011. Biological and molecular characterization of Beet oak-leaf virus. American Society of Sugarbeet Technologists. Online.
Technical Abstract: Beet oak-leaf virus (BOLV) was first isolated from Rhizomania infested fields in California in early 2000. The infected sugar beet leaves showed oak-leaf pattern symptoms in some breeding lines different from Rhizomania, while some beet cultivars were symptomless. BOLV is transmitted by Polymyxe betae and also can be transmitted by rub inoculations. The host range of BOLV is similar to Beet necrotic yellow vein virus (BNYVV) and Beet soil-borne mosaic virus (BSBMV), which mainly infects Chenopodiaceous plants. BOLV has been purified from rub inoculated spinach (Spinacia oleracea) plants. The particle morphology is identical to BNYVV. The molecular mass of the capsid protein was estimated to be 46.0 kDa. A polyclonal antibody from rabbits has been produced from purified BOLV virions and can be used in enzyme-linked immunosorbent assay (ELISA), western blot, and immunogold labeling tests. BOLV is serologically distinct from BNYVV, BSBMV, and Beet soil-borne virus (BSBV). BOLV has also been found in Colorado, Michigan, Minnesota, Nebraska, and Wyoming. BOLV was found in sugar beet alone or co-infected with BNYVV or BSBMV. The interaction tests between BNYVV and BOLV indicate that BOLV may suppress BNYVV in mixed infections.