|LI, YUHONG - University Of Wisconsin|
Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2010
Publication Date: 12/15/2010
Citation: Li, Y., Weng, Y. 2010. Molecular mapping of genes for compact growth habit and fruit spine color in cultivated cucumber (Cucumis sativus var. sativus L.) [abstract]. Plant and Animal Genome Conference. P324.
Technical Abstract: The compact (dwarf) plant architecture and fruit spine color are two important traits in cucumber breeding, which are controlled by simply inherited recessive (cp) and dominant (B) genes, respectively. In the present study, linkage analysis of molecular markers with the two traits was conducted with an F2:3 mapping population that is segregating at both cp and B loci, which was developed from a cross between PI 308915 (compact plant architecture with white spine, light green mature fruit) and PI 249561 (regular plant architecture with black spine, yellow mature fruit). From 2,212 cucumber SSRs, 245 polymorphic markers (11.1% polymorphism) were identified and applied in 46 F2 plants, and 205 markers were finally mapped. Linkage analysis placed both cp and B at the opposite ends of cucumber Chromosome 4. Based on whole genome sequence information, more closely linked SSR or SNP markers were identified with a stepwise increase of mapping population size. So far, flanking molecular markers within 0.5 cM genetic distances for both targets genes and their associated scaffolds have been identified. Since no recombination was observed between black spine and yellow mature fruit color, molecular markers for black spine were also applicable for yellow mature fruit color.