Location: Location not imported yet.Title: The penultimate arginine of the carboxy terminus determines slow desensitisation in a P2X receptor from the cattle tick Boophilus microplus Author
|Guerrero, Felicito - Felix|
Submitted to: Molecular Pharmacology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/6/2011
Publication Date: 4/20/2011
Publication URL: http://handle.nal.usda.gov/10113/55691
Citation: Bavan, S., Farmer, L., Singh, S.K., Straub, V.A., Guerrero, F., Ennion, S.J. 2011. The penultimate arginine of the carboxy terminus determines slow desensitisation in a P2X receptor from the cattle tick Boophilus microplus. Molecular Pharmacology. 79(4):776-785. Interpretive Summary: P2X receptors are extracellular ATP-gated ion channels that exist in eukaryotic organisms. The P2X receptor of the cattle tick is classified as a slow desensitizing channel, while the human P2X receptor is classified as a rapidly desensitizing channel. The activity of these channels was investigated by exchanging the N- or C- termini of the cattle tick and human receptors, expressing the chimeric receptors in Xenopus oocytes, and using electrophysiology to characterize the desensitizing activity. The results showed that the penultimate arginine of the carboxy terminus of the cattle tick receptor was responsible for the slow desensitizing activity. Mutation of this positively charged arginine to an uncharged amino acid resulted in significantly faster desensitization of the cattle tick P2X receptor.
Technical Abstract: P2X ion channels have been functionally characterised from a range of eukaryotes. Whilst these receptors can be broadly classified into fast and slow desensitising, the molecular mechanisms underlying current desensitisation are not fully understood. Here we describe the characterisation of a P2X channel from the cattle tick Boophilus microplus (BmP2X) displaying extremely slow current kinetics, little desensitisation during ATP application and marked run-down in current amplitude between sequential responses. ATP (EC50 67.1 µM) evoked concentration dependent currents at BmP2X which were antagonised by suramin (IC50 4.8 µM) and potentiated by the antiparasitic drug amitraz. Ivermectin did not potentiate BmP2X currents but the mutation M362L conferred ivermectin sensitivity. To investigate the mechanisms underlying slow desensitisation we generated intracellular domain chimeras between BmP2X and the rapidly desensitising HdP2X receptor from Hypsibius dujardini. Exchange of N- or C-termini between these fast and slow desensitising receptors altered the rate of current desensitisation towards that of the donor channel. Truncation of the BmP2X C-terminus identified the penultimate residue (R413) as important for slow desensitisation. Removal of positive charge at this position in the mutant R413A resulted in significantly faster desensitisation which was further accentuated by the negatively charged substitution R413D. R413A and R413D however still displayed current run-down to sequential ATP application. Mutation to a positive charge (R413K) reconstituted the wild-type phenotype. This study identifies a new paradigm by which positive charge in the C-terminal domain regulates P2X receptor desensitisation during ATP application and further demonstrates that run-down and desensitisation are governed by distinct mechanisms.